Longname
Mammalian sterile 20-like 4
3Letter
MT4
Accession
NM_016542.3
Activation
N/A constitutive
Assay Method
5-20mU of MST4 (diluted in 50mM Tris (pH 7.5), 0.1mM EGTA) is assayed against MBP in a final volume of 25.5µl containing 50mM Tris (pH 7.5), 0.1mM EGTA, 0.33mg/ml MBP, 10 mM magnesium acetate and 0.02 mM [33P-γ-ATP]( 500-1000 cpm/pmole) and incubated for 30 mins at room temperature. Assays are stopped by addition of 5µl of 0.5M (3%) orthophosphoric acid. Assays are harvested onto P81 Unifilter plates using a wash buffer of 50mM orthophosphoric acid.
ATPGroup
20
Base36
2B
Express
Off
ExpressionSys
Sf21 cells
KCode
83
Kinase Type
k
PDF
MST4_5.pdf
(76.16 KB)
Species
Human
TissRec
R