Longname
Myristoylated and Palmitoylated serine/threonine protein Kinase,also know as STK16
3Letter
S16
Accession
CR407675
Activation
N/A constitutive
Assay Method
MPSK1(5-20mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 1 mg/ml BSA 0.1%,βmercaptoethanol) is assayed against asubstrate peptide (RRHYYYDTHTNTYYLRTFGHNTRR) in a final volume of 25.5 μl containing 50mM Tris pH 7.5, 0.1mMEGTA, 10mM MnCl,300μM substrate peptide, 10 mM magnesium acetate 0.05 mM [33P-γ-ATP] (50-1000cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 μl of0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50mM orthophosphoric acid.
ATPGroup
50
Base36
4D
Express
Off
ExpressionSys
E.coli
KCode
157
Kinase Type
k
PDF
MPSK1_screening.pdf
(87.48 KB)
Species
Human
TissRec
R