Longname
MAP kinase interacting Kinase 2α
3Letter
MN2
Accession
AF237775
Activation
SAPK2a/p38
Assay Method
5-20mU of MNK2α (diluted in 50mM Tris (pH 7.5), 0.1mM EGTA, 1mg/ml BSA, 0.1%,β-mercaptoethanol) is assayed against substrate peptide (eIF4E) in a final volume of 25.5µl containing 50mM Tris (pH 7.5), 0.1mM EGTA, 0.1%, β-mercaptoethanol, 0.5mg/ml substrate peptide, 10 mM magnesium acetate and 0.05 mM [33P-γ-ATP]( 500-1000 cpm/pmole) and incubated for 30 mins at room temperature. Assays are stopped by addition of 5µl of 0.5M (3%) orthophosphoric acid. Assays are harvested onto P81 Unifilter plates using a wash buffer of 50mM orthophosphoric acid.
ATPGroup
50
Base36
18
Express
Off
ExpressionSys
E. coli
KCode
44
Kinase Type
k
PDF
MNK2_5.pdf
(83.41 KB)
Species
Human
TissRec
R