Inhibitor | Akti 1/2  

Screen Conc: 100.00µM

Kinase	% Activity Remaining Sort descending	Standard Deviation
SmMLCK	-1	0
PKB alpha	6	0
CAMK1	8	0
MAPKAP-K2	12	0
MKK1	14	3
SGK1	14	5
NEK7	15	0
PIM3	16	1
PIM1	17	1
RSK2	22	1
PIM2	23	0
PKB beta	26	2
Lck	31	3
PAK4	33	1
S6K1	34	11
RSK1	36	1
PKA	39	0
MSK1	41	1
JNK3	42	1
EF2K	43	1
GSK3 beta	43	9
SYK	44	2
NEK6	44	1
ERK2	45	1
MELK	45	5
JNK1	48	1
ERK8	51	1
PRAK	51	4
MAPKAP-K3	52	2
FGF-R1	55	0
Aurora B	56	11
CHK2	58	3
PRK2	59	2
PHK	59	2
DYRK3	60	4
HIPK2	61	1
YES1	61	2
Src	62	5
PAK5	63	5
PAK6	66	1
SRPK1	67	0
IRR	68	1
HIPK3	68	2
EPH-A2	69	1
Aurora C	70	15
NEK2a	70	2
BRSK2	76	9
MNK2	77	10
JNK2	80	7
PKD1	81	3
MNK1	82	0
PLK1	83	2
MST2	83	7
p38 alpha MAPK	83	2
CK2	84	7
p38 beta MAPK	86	15
p38 gamma MAPK	87	0
ERK1	87	2
CK1 delta	88	5
CHK1	88	5
p38 delta MAPK	89	3
MARK3	90	8
CSK	92	0
MST4	92	6
ROCK 2	92	9
CDK2-Cyclin A	93	3
PKC alpha	96	3
AMPK	97	0
IKK beta	98	2
PDK1	98	15
DYRK1A	99	5
TBK1	100	4
IKK epsilon	100	1
PKC zeta	104	8
DYRK2	106	0

Screen Conc: 10.00µM

Kinase	% Activity Remaining Sort descending	Standard Deviation
CAMK1	3	0
SmMLCK	11	4
EF2K	40	6
PAK4	48	4
PIM1	56	6
PIM3	59	6
MAPKAP-K2	59	1
Aurora B	60	3
PAK5	60	3
CAMKK alpha	62	3
Lck	64	6
PKB alpha	64	8
Aurora C	65	0
HIPK3	65	2
RSK1	67	1
IKK epsilon	67	6
PRK2	67	10
MELK	67	0
ERK8	70	1
JNK1	70	9
S6K1	71	6
MAPKAP-K3	71	5
DYRK3	74	1
RSK2	74	1
ERK2	75	14
NEK6	75	3
FGF-R1	75	3
MKK1	76	10
PKA	76	5
GSK3 beta	77	9
MST2	78	2
MNK2	79	3
PRAK	79	5
MST4	80	4
JNK3	80	7
JNK2	81	12
IRR	81	13
PKD1	82	14
SGK1	82	3
CHK2	82	7
EPH-A2	84	7
MNK1	84	1
SYK	84	10
SRPK1	84	9
NEK7	84	10
HIPK2	84	3
CHK1	85	2
PKB beta	87	14
PKC alpha	87	8
YES1	88	8
PLK1	89	2
PIM2	89	3
IKK beta	89	2
CK2	91	14
ROCK 2	91	1
PAK6	92	1
AMPK	92	10
BRSK2	92	12
p38 gamma MAPK	93	9
MSK1	93	14
DYRK1A	93	12
PDK1	94	7
MARK3	95	12
PKC zeta	96	9
CDK2-Cyclin A	97	13
p38 delta MAPK	98	11
p38 alpha MAPK	98	13
ERK1	99	5
NEK2a	99	3
PHK	101	9
CSK	103	15
TBK1	106	10
CAMKK beta	108	9
CK1 delta	110	2
p38 beta MAPK	113	3
DYRK2	118	1
Src	118	1

Screen Conc: 1.00µM

Kinase	% Activity Remaining Sort descending	Standard Deviation
CAMK1	44	7
MINK1	54	9
RIPK2	57	3
EF2K	61	6
PIM3	63	1
PAK5	63	1
PKB beta	65	7
MST2	66	1
PAK4	68	9
NUAK1	71	0
VEGFR1	71	3
MLK1	73	0
RSK2	74	1
BTK	75	2
TrkA	76	7
IKK epsilon	76	3
HIPK1	77	2
TAK1	78	8
MELK	79	2
YES1	79	6
PRK2	79	7
MARK1	79	1
PIM1	80	0
FGF-R1	80	7
HIPK2	81	4
SmMLCK	82	13
p38 alpha MAPK	82	1
ROCK 2	82	10
PKC alpha	82	3
MST4	83	2
MKK1	84	12
DYRK1A	84	2
SRPK1	84	7
PKD1	86	7
PIM2	86	1
TAO1	87	4
AMPK	87	6
ERK8	87	4
DYRK2	88	5
p38 delta MAPK	88	8
MAPKAP-K3	88	2
TBK1	88	4
SYK	89	7
MLK3	89	4
LKB1	89	0
CHK2	89	11
GCK	89	2
JNK1	90	2
NEK2a	90	3
Lck	90	4
DYRK3	91	11
DAPK1	91	4
MAPKAP-K2	91	3
PAK2	92	8
TTK	92	3
ERK1	92	8
JNK2	92	11
PRAK	92	5
IKK beta	93	8
MARK2	93	3
IRAK4	93	6
MSK1	94	4
Aurora A	94	4
BRSK1	94	4
IRR	94	3
CK1 delta	94	0
PKB alpha	95	4
HER4	95	4
BRSK2	95	5
EPH-B4	95	13
HIPK3	95	8
CLK2	96	10
IGF-1R	96	0
EPH-B3	96	14
p38 gamma MAPK	97	3
RSK1	97	4
MARK3	98	5
PLK1	98	4
MEKK1	98	2
SGK1	98	4
CHK1	98	10
Aurora B	100	10
EPH-B1	100	8
PKA	100	10
MNK2	100	2
EPH-B2	100	8
MKK2	101	3
MKK6	101	11
EPH-A4	101	1
PHK	102	11
CSK	102	2
MARK4	103	3
EPH-A2	103	6
ERK2	103	4
GSK3 beta	103	1
JAK2	103	2
CAMKK beta	104	1
JNK3	104	9
Src	105	9
S6K1	106	8
PDK1	108	4
CDK2-Cyclin A	108	14
p38 beta MAPK	108	9
IR	109	3
PAK6	109	8
ASK1	109	3
MNK1	110	2
CK2	111	0
PKC zeta	113	4
NEK6	117	8

Screen Conc: 0.10µM

Kinase	% Activity Remaining Sort descending	Standard Deviation
ERK2	71	15
HIPK3	72	2
MKK1	75	2
MAPKAP-K3	76	3
PRK2	78	1
JNK3	79	3
ERK8	80	5
MNK1	81	0
Lck	81	4
NEK6	83	1
CHK2	83	7
GSK3 beta	83	3
PIM1	83	12
PLK1	83	2
SYK	83	2
Aurora B	83	5
Aurora C	83	0
MAPKAP-K2	84	6
MSK1	84	2
JNK1	85	0
RSK2	85	7
CK2	85	1
PKB alpha	85	3
MARK3	86	1
MELK	86	10
SmMLCK	86	7
NEK7	86	0
YES1	86	4
PAK5	87	7
DYRK3	87	6
SRPK1	87	1
FGF-R1	89	8
CAMK1	90	3
PKA	90	8
PKD1	90	4
PDK1	91	9
IKK beta	91	11
IRR	91	3
NEK2a	92	6
ROCK 2	92	7
CDK2-Cyclin A	92	3
MST4	92	1
MNK2	93	11
BRSK2	93	0
PRAK	93	5
RSK1	94	4
JNK2	95	0
EPH-A2	95	6
PIM2	95	6
ERK1	95	3
AMPK	96	14
DYRK1A	97	11
Src	97	3
CSK	97	11
CHK1	97	0
PIM3	97	5
HIPK2	98	6
S6K1	98	1
PAK4	99	4
PKB beta	101	6
PKC alpha	101	5
PKC zeta	101	0
TBK1	103	11
p38 beta MAPK	103	10
MST2	103	0
PHK	104	1
p38 delta MAPK	104	5
DYRK2	105	3
CK1 delta	106	7
SGK1	106	5
EF2K	107	11
IKK epsilon	107	7
p38 gamma MAPK	110	7
p38 alpha MAPK	111	5
PAK6	112	8