Inhibitor | PLX 4720 (Raf Kinase Inhibitor V)  

Screen Conc: 0.10µM

Kinase	% Activity Remaining	Standard Deviation
AMPK	91	17
Aurora B	86	0
BRSK2	109	4
BTK	102	4
CAMK1	107	13
CAMKK beta	92	1
CDK2-Cyclin A	80	12
CHK1	87	6
CHK2	78	19
CK1 delta	92	0
CK2	88	25
CSK	95	18
DYRK1A	96	2
DYRK2	87	16
DYRK3	97	4
EF2K	97	6
EPH-A2	100	2
EPH-B3	105	18
ERK1	115	44
ERK2	81	17
ERK8	91	10
FGF-R1	101	0
GSK3 beta	95	3
HIPK2	92	8
IGF-1R	90	3
IKK beta	100	14
IKK epsilon	84	3
IR	99	0
IRR	88	10
JNK1	104	5
JNK2	90	9
Lck	77	7
MAPKAP-K2	102	12
MARK3	92	8
MELK	81	7
MKK1	116	28
MNK1	82	6
MNK2	77	5
MSK1	90	10
MST2	85	6
MST4	104	3
NEK2a	84	13
NEK6	94	2
p38 alpha MAPK	84	13
p38 beta MAPK	85	13
p38 delta MAPK	106	8
p38 gamma MAPK	95	8
PAK4	79	5
PAK5	119	8
PAK6	101	13
PDK1	96	1
PHK	97	22
PIM1	106	11
PIM2	115	13
PIM3	109	9
PKA	94	6
PKB alpha	92	13
PKB beta	100	10
PKC alpha	96	2
PKC zeta	102	6
PKD1	97	2
PLK1	94	0
PRAK	98	11
PRK2	93	5
ROCK 2	99	1
RSK1	105	0
RSK2	93	17
S6K1	93	13
SGK1	88	9
SmMLCK	93	1
Src	71	4
SRPK1	89	8
SYK	98	1
TBK1	94	9
VEGFR1	87	3
YES1	74	4

Screen Conc: 1.00µM

Kinase	% Activity Remaining	Standard Deviation
AMPK	114	2
ASK1	100	7
Aurora A	84	0
Aurora B	71	8
BRSK1	97	3
BRSK2	107	10
BTK	116	12
CAMK1	84	2
CAMKK beta	98	7
CDK2-Cyclin A	110	4
CHK1	81	5
CHK2	115	14
CK1 delta	98	3
CK2	109	10
CLK2	84	14
CSK	59	2
DAPK1	98	3
DYRK1A	99	10
DYRK2	106	2
DYRK3	98	6
EF2K	122	11
EPH-A2	105	2
EPH-A4	113	5
EPH-B1	94	0
EPH-B2	114	0
EPH-B3	126	13
EPH-B4	125	1
ERK1	116	0
ERK2	112	6
ERK8	88	3
FGF-R1	80	14
GCK	94	8
GSK3 beta	97	12
HER4	96	0
HIPK1	109	1
HIPK2	95	3
HIPK3	104	4
IGF-1R	110	9
IKK beta	89	3
IKK epsilon	102	8
IR	116	0
IRAK4	107	1
IRR	95	11
JAK2	95	11
JNK1	106	3
JNK2	98	0
JNK3	75	7
Lck	58	8
LKB1	109	5
MAPKAP-K2	112	12
MAPKAP-K3	115	5
MARK1	97	0
MARK2	93	3
MARK3	93	15
MARK4	98	1
MEKK1	96	2
MELK	116	10
MINK1	105	13
MKK1	84	8
MKK2	79	2
MKK6	125	11
MLK1	88	10
MLK3	84	7
MNK1	107	7
MNK2	113	7
MSK1	111	3
MST2	76	13
MST4	109	1
NEK2a	98	8
NEK6	112	3
NUAK1	83	6
p38 alpha MAPK	83	2
p38 beta MAPK	88	3
p38 delta MAPK	92	9
p38 gamma MAPK	108	5
PAK2	83	5
PAK4	77	1
PAK5	108	2
PAK6	107	13
PDK1	94	0
PHK	111	3
PIM1	104	13
PIM2	102	1
PIM3	97	3
PKA	112	1
PKB alpha	105	3
PKB beta	82	14
PKC alpha	104	7
PKC zeta	101	2
PKD1	93	15
PLK1	126	4
PRAK	93	5
PRK2	99	2
RIPK2	21	7
ROCK 2	91	3
RSK1	92	2
RSK2	111	7
S6K1	93	1
SGK1	103	4
SmMLCK	90	4
Src	62	3
SRPK1	101	4
SYK	114	9
TAK1	96	11
TAO1	109	9
TBK1	102	3
TrkA	107	12
TTK	88	1
VEGFR1	49	4
YES1	43	2

Screen Conc: 10.00µM

Kinase	% Activity Remaining	Standard Deviation
AMPK	56	4
ASK1	95	15
Aurora A	23	1
Aurora B	20	4
BRSK1	82	6
BRSK2	73	8
BTK	69	0
CAMK1	27	1
CAMKK beta	71	6
CDK2-Cyclin A	102	3
CHK1	28	0
CHK2	77	0
CK1 delta	96	4
CK2	77	3
CLK2	20	2
CSK	84	2
DAPK1	83	13
DYRK1A	77	6
DYRK2	81	4
DYRK3	68	1
EF2K	100	2
EPH-A2	97	2
EPH-A4	96	3
EPH-B1	85	3
EPH-B2	76	1
EPH-B3	83	12
EPH-B4	97	0
ERK1	103	7
ERK2	90	6
ERK8	42	3
FGF-R1	30	3
GCK	26	2
GSK3 beta	53	3
HER4	49	3
HIPK1	93	3
HIPK2	48	7
HIPK3	90	14
IGF-1R	80	3
IKK beta	71	3
IKK epsilon	81	10
IR	117	9
IRAK4	87	4
IRR	64	6
JAK2	45	1
JNK1	89	1
JNK2	88	6
JNK3	85	4
Lck	34	6
LKB1	64	4
MAPKAP-K2	100	7
MAPKAP-K3	89	2
MARK1	70	0
MARK2	87	2
MARK3	74	0
MARK4	65	3
MEKK1	65	3
MELK	24	0
MINK1	15	0
MKK1	45	1
MKK2	25	3
MKK6	94	10
MLK1	34	4
MLK3	33	3
MNK1	79	13
MNK2	98	10
MSK1	91	3
MST2	40	1
MST4	66	4
NEK2a	52	0
NEK6	114	13
NUAK1	61	7
p38 alpha MAPK	58	6
p38 beta MAPK	57	2
p38 delta MAPK	79	6
p38 gamma MAPK	94	2
PAK2	40	8
PAK4	46	1
PAK5	49	4
PAK6	79	2
PDK1	85	12
PHK	110	1
PIM1	68	5
PIM2	92	3
PIM3	61	3
PKA	104	1
PKB alpha	101	6
PKB beta	78	15
PKC alpha	84	4
PKC zeta	93	7
PKD1	87	13
PLK1	95	1
PRAK	93	2
PRK2	89	12
RIPK2	7	1
ROCK 2	66	1
RSK1	98	4
RSK2	80	3
S6K1	84	1
SGK1	88	11
SmMLCK	36	1
Src	21	0
SRPK1	79	3
SYK	96	14
TAK1	92	9
TAO1	63	3
TBK1	96	7
TrkA	54	4
TTK	75	11
VEGFR1	10	0
YES1	13	3