Inhibitor | Volasertib  

Screen Conc: 0.10µM

Kinase	% Activity Remaining	Standard Deviation
ABL	97	4
AMPK (hum)	101	2
ASK1	93	16
Aurora A	88	7
Aurora B	89	5
BRK	103	7
BRSK1	93	3
BRSK2	78	2
BTK	101	5
CAMK1	90	11
CAMKK beta	61	4
CDK2-Cyclin A	90	7
CDK9-Cyclin T1	82	4
CHK1	93	9
CHK2	103	1
CK1 delta	107	10
CK1 gamma 2	108	2
CK2	107	6
CLK2	82	19
CSK	83	6
DAPK1	96	4
DDR2	109	27
DYRK1A	87	3
DYRK2	85	17
DYRK3	100	15
EF2K	101	9
EIF2AK3	85	1
EPH-A2	116	1
EPH-A4	99	5
EPH-B1	93	4
EPH-B2	128	19
EPH-B3	123	14
EPH-B4	92	4
ERK1	104	11
ERK2	98	13
ERK5	88	1
ERK8	110	2
FGF-R1	92	3
GCK	87	16
GSK3 beta	98	12
HER4	93	1
HIPK1	99	8
HIPK2	112	12
HIPK3	97	5
IGF-1R	104	19
IKK beta	89	3
IKK epsilon	87	5
IR	116	21
IRAK1	90	1
IRAK4	80	10
IRR	105	0
JAK2	105	10
JNK1	106	5
JNK2	99	20
JNK3	91	6
Lck	115	16
LKB1	89	4
MAP4K3	81	8
MAP4K5	84	6
MAPKAP-K2	90	6
MAPKAP-K3	94	4
MARK1	87	1
MARK2	94	3
MARK3	102	8
MARK4	103	11
MEKK1	106	9
MELK	93	6
MINK1	93	17
MKK1	86	6
MKK2	92	20
MKK6	79	16
MLK1	79	4
MLK3	96	7
MNK1	92	8
MNK2	107	2
MPSK1	89	36
MSK1	88	1
MST2	82	15
MST3	88	1
MST4	96	17
NEK2a	85	10
NEK6	120	6
NUAK1	95	8
OSR1	91	3
p38 alpha MAPK	96	17
p38 beta MAPK	116	4
p38 delta MAPK	97	17
p38 gamma MAPK	91	4
PAK2	89	3
PAK4	83	11
PAK5	106	3
PAK6	93	2
PDGFRA	115	12
PDK1	87	4
PHK	107	11
PIM1	90	1
PIM2	100	8
PIM3	99	12
PINK	96	12
PKA	104	9
PKB alpha	108	9
PKB beta	96	14
PKC alpha	105	11
PKC gamma	107	8
PKC zeta	95	18
PKD1	78	7
PLK1	4	1
PRAK	90	8
PRK2	114	15
RIPK2	99	19
ROCK 2	82	10
RSK1	89	9
RSK2	95	18
S6K1	88	0
SGK1	91	7
SIK2	84	2
SIK3	106	13
SmMLCK	75	22
Src	89	9
SRPK1	113	5
STK33	87	0
SYK	112	7
TAK1	96	12
TAO1	88	13
TBK1	105	19
TESK1	100	29
TGFBR1	97	2
TIE2	81	9
TLK1	91	1
TrkA	83	16
TSSK1	106	13
TTBK1	99	0
TTBK2	114	17
TTK	93	4
ULK1	89	9
ULK2	121	21
VEGFR1	98	13
WNK1	89	2
YES1	105	9
ZAP70	88	3

Screen Conc: 1.00µM

Kinase	% Activity Remaining	Standard Deviation
ABL	83	12
AMPK (hum)	97	13
ASK1	93	3
Aurora A	85	7
Aurora B	85	11
BRK	90	17
BRSK1	82	0
BRSK2	71	6
BTK	106	2
CAMK1	91	8
CAMKK beta	13	1
CDK2-Cyclin A	92	5
CDK9-Cyclin T1	78	14
CHK1	104	3
CHK2	67	8
CK1 delta	108	12
CK1 gamma 2	88	13
CK2	106	15
CLK2	36	6
CSK	87	2
DAPK1	77	9
DDR2	104	23
DYRK1A	95	11
DYRK2	72	8
DYRK3	87	6
EF2K	97	4
EIF2AK3	58	4
EPH-A2	97	5
EPH-A4	90	0
EPH-B1	91	7
EPH-B2	122	0
EPH-B3	103	5
EPH-B4	97	1
ERK1	91	7
ERK2	104	8
ERK5	87	2
ERK8	109	2
FGF-R1	93	3
GCK	71	2
GSK3 beta	93	10
HER4	87	12
HIPK1	102	8
HIPK2	98	10
HIPK3	95	3
IGF-1R	87	30
IKK beta	93	4
IKK epsilon	92	20
IR	93	8
IRAK1	79	14
IRAK4	92	5
IRR	89	6
JAK2	83	18
JNK1	101	3
JNK2	93	14
JNK3	91	16
Lck	96	8
LKB1	99	4
MAP4K3	68	1
MAP4K5	85	23
MAPKAP-K2	95	10
MAPKAP-K3	100	12
MARK1	87	23
MARK2	91	18
MARK3	105	10
MARK4	96	3
MEKK1	95	18
MELK	68	22
MINK1	79	1
MKK1	80	5
MKK2	72	16
MKK6	89	25
MLK1	83	1
MLK3	99	24
MNK1	98	7
MNK2	103	4
MPSK1	72	20
MSK1	99	6
MST2	88	7
MST3	101	8
MST4	89	10
NEK2a	67	8
NEK6	111	25
NUAK1	92	17
OSR1	59	1
p38 alpha MAPK	95	2
p38 beta MAPK	107	1
p38 delta MAPK	99	5
p38 gamma MAPK	98	3
PAK2	91	25
PAK4	94	12
PAK5	105	10
PAK6	105	10
PDGFRA	108	10
PDK1	87	4
PHK	87	9
PIM1	94	9
PIM2	97	10
PIM3	80	18
PINK	93	3
PKA	97	1
PKB alpha	95	3
PKB beta	104	3
PKC alpha	103	7
PKC gamma	96	3
PKC zeta	91	0
PKD1	49	5
PLK1	2	1
PRAK	93	3
PRK2	98	19
RIPK2	100	11
ROCK 2	94	8
RSK1	69	7
RSK2	82	13
S6K1	89	11
SGK1	94	9
SIK2	89	14
SIK3	97	4
SmMLCK	33	10
Src	90	14
SRPK1	101	3
STK33	82	6
SYK	89	2
TAK1	94	4
TAO1	98	1
TBK1	97	4
TESK1	93	8
TGFBR1	112	31
TIE2	82	0
TLK1	95	24
TrkA	75	3
TSSK1	88	1
TTBK1	102	6
TTBK2	102	8
TTK	82	6
ULK1	92	4
ULK2	103	11
VEGFR1	96	16
WNK1	101	5
YES1	93	5
ZAP70	85	12