Inhibitor | Celastrol  

Screen Conc: 1.00µM

Kinase	% Activity Remaining	Standard Deviation
ABL	93	0
AMPK (hum)	91	5
ASK1	106	1
Aurora A	96	0
Aurora B	67	7
BRK	101	5
BRSK1	106	13
BRSK2	65	0
BTK	108	6
CAMK1	86	8
CAMKK beta	82	4
CDK2-Cyclin A	84	1
CDK9-Cyclin T1	87	17
CHK1	94	8
CHK2	99	3
CK1 delta	88	7
CK1 gamma 2	103	6
CK2	97	6
CLK2	92	9
CSK	79	0
DAPK1	88	17
DDR2	116	13
DYRK1A	101	4
DYRK2	71	10
DYRK3	89	9
EF2K	87	8
EIF2AK3	87	6
EPH-A2	99	11
EPH-A4	98	14
EPH-B1	78	1
EPH-B2	98	6
EPH-B3	92	22
EPH-B4	99	9
ERK1	95	16
ERK2	86	12
ERK5	82	0
ERK8	62	5
FGF-R1	78	7
GCK	73	1
GSK3 beta	99	2
HER4	100	7
HIPK1	94	11
HIPK2	96	6
HIPK3	97	4
IGF-1R	117	8
IKK beta	101	10
IKK epsilon	99	8
IR	96	15
IRAK1	93	5
IRAK4	108	11
IRR	93	8
JAK2	89	6
JNK1	106	2
JNK2	96	23
JNK3	76	2
Lck	93	10
LKB1	83	3
MAP4K3	92	1
MAP4K5	92	10
MAPKAP-K2	104	11
MAPKAP-K3	98	3
MARK1	89	3
MARK2	86	6
MARK3	96	2
MARK4	92	7
MEKK1	103	1
MELK	88	27
MINK1	97	9
MKK1	89	4
MKK2	89	2
MKK6	111	0
MLK1	85	6
MLK3	66	3
MNK1	89	8
MNK2	91	10
MPSK1	98	11
MSK1	90	3
MST2	111	12
MST3	110	3
MST4	109	8
NEK2a	102	3
NEK6	88	6
NUAK1	96	17
OSR1	116	22
p38 alpha MAPK	93	3
p38 beta MAPK	102	1
p38 delta MAPK	80	6
p38 gamma MAPK	71	18
PAK2	100	2
PAK4	98	13
PAK5	103	12
PAK6	96	12
PDGFRA	100	2
PDK1	97	9
PHK	92	3
PIM1	92	3
PIM2	108	1
PIM3	110	6
PINK	97	12
PKA	99	0
PKB alpha	100	28
PKB beta	89	1
PKC alpha	92	7
PKC gamma	63	12
PKC zeta	115	11
PKD1	79	1
PLK1	92	7
PRAK	63	5
PRK2	93	13
RIPK2	94	8
ROCK 2	83	5
RSK1	81	2
RSK2	72	8
S6K1	101	7
SGK1	88	0
SIK2	85	4
SIK3	94	16
SmMLCK	83	5
Src	110	3
SRPK1	92	2
STK33	95	4
SYK	81	19
TAK1	85	7
TAO1	100	13
TBK1	99	11
TESK1	90	6
TGFBR1	96	8
TIE2	93	4
TLK1	81	5
TrkA	74	10
TSSK1	60	6
TTBK1	113	2
TTBK2	115	5
TTK	83	3
ULK1	99	16
ULK2	107	12
VEGFR1	77	18
WNK1	91	8
YES1	101	9
ZAP70	78	10

Screen Conc: 5.00µM

Kinase	% Activity Remaining	Standard Deviation
ABL	98	8
AMPK (hum)	74	6
ASK1	133	8
Aurora A	93	3
Aurora B	11	2
BRK	113	5
BRSK1	81	0
BRSK2	23	1
BTK	106	11
CAMK1	103	2
CAMKK beta	108	1
CDK2-Cyclin A	83	5
CDK9-Cyclin T1	102	3
CHK1	129	8
CHK2	82	1
CK1 delta	130	43
CK1 gamma 2	96	3
CK2	103	4
CLK2	91	1
CSK	89	1
DAPK1	97	1
DDR2	110	6
DYRK1A	96	13
DYRK2	88	23
DYRK3	23	6
EF2K	111	7
EIF2AK3	99	2
EPH-A2	88	17
EPH-A4	90	10
EPH-B1	133	33
EPH-B2	116	24
EPH-B3	112	28
EPH-B4	121	10
ERK1	107	3
ERK2	39	1
ERK5	96	11
ERK8	58	1
FGF-R1	63	8
GCK	89	6
GSK3 beta	115	1
HER4	140	5
HIPK1	89	0
HIPK2	106	4
HIPK3	77	13
IGF-1R	110	17
IKK beta	74	3
IKK epsilon	67	4
IR	91	4
IRAK1	98	6
IRAK4	142	7
IRR	83	6
JAK2	103	1
JNK1	107	10
JNK2	101	23
JNK3	82	11
Lck	104	10
LKB1	94	6
MAP4K3	50	14
MAP4K5	108	1
MAPKAP-K2	113	2
MAPKAP-K3	64	1
MARK1	76	6
MARK2	88	8
MARK3	92	6
MARK4	57	2
MEKK1	108	1
MELK	93	7
MINK1	104	15
MKK1	88	1
MKK2	71	4
MKK6	120	1
MLK1	89	8
MLK3	30	5
MNK1	117	2
MNK2	107	10
MPSK1	120	8
MSK1	95	3
MST2	95	1
MST3	122	2
MST4	82	6
NEK2a	118	8
NEK6	78	1
NUAK1	84	3
OSR1	138	4
p38 alpha MAPK	105	6
p38 beta MAPK	95	0
p38 delta MAPK	119	0
p38 gamma MAPK	107	11
PAK2	94	1
PAK4	86	7
PAK5	83	3
PAK6	113	6
PDGFRA	103	3
PDK1	89	1
PHK	100	4
PIM1	99	1
PIM2	111	14
PIM3	98	6
PINK	81	8
PKA	115	9
PKB alpha	91	17
PKB beta	82	3
PKC alpha	102	0
PKC gamma	52	6
PKC zeta	174	7
PKD1	75	5
PLK1	68	18
PRAK	45	8
PRK2	85	4
RIPK2	121	4
ROCK 2	103	9
RSK1	84	2
RSK2	60	7
S6K1	70	5
SGK1	80	3
SIK2	69	6
SIK3	64	2
SmMLCK	104	5
Src	90	2
SRPK1	96	5
STK33	97	1
SYK	131	6
TAK1	83	4
TAO1	106	12
TBK1	116	1
TESK1	91	24
TGFBR1	97	9
TIE2	98	10
TLK1	94	5
TrkA	66	12
TSSK1	42	1
TTBK1	110	2
TTBK2	91	13
TTK	71	4
ULK1	103	1
ULK2	104	16
VEGFR1	49	6
WNK1	97	2
YES1	99	7
ZAP70	114	4