Inhibitor | Chelerythrine Chloride  

Screen Conc: 10.00µM

Kinase	% Activity Remaining Sort descending	Standard Deviation
MLK3	1	0
MINK1	6	0
VEGFR1	10	3
NEK6	10	0
CAMK1	12	3
MLK1	13	1
NEK2a	18	0
TIE2	18	1
IKK beta	20	8
PKB beta	22	2
IKK epsilon	22	1
HIPK3	22	1
GCK	27	2
IRAK4	27	5
ERK8	28	1
FGF-R1	29	3
DYRK3	31	1
HIPK2	32	8
BTK	36	2
Src	40	7
BRSK1	41	9
MAPKAP-K3	45	7
YES1	45	8
RSK2	47	6
SYK	48	2
PKC gamma	51	2
PIM1	53	2
TrkA	53	4
PLK1	57	1
JNK1	57	1
HIPK1	58	1
HER4	61	3
JNK3	61	6
CAMKK beta	61	3
Aurora A	62	6
PIM3	64	4
LKB1	64	1
TAK1	64	6
MKK1	65	6
DYRK2	65	1
MARK4	66	4
CLK2	66	1
AMPK	67	4
PKD1	67	2
BRSK2	68	8
DYRK1A	68	7
NUAK1	69	9
PKC zeta	69	0
PRAK	69	0
JAK2	70	7
MEKK1	70	1
TBK1	70	1
SRPK1	71	2
SmMLCK	71	5
p38 alpha MAPK	71	1
MKK2	72	1
TTK	73	4
PAK4	74	3
MSK1	74	1
PAK6	74	8
ROCK 2	74	0
MARK2	75	7
MST2	75	0
PIM2	75	5
PKB alpha	75	2
RSK1	77	3
EPH-A2	78	4
ABL	78	6
PAK2	79	7
RIPK2	79	1
STK33	79	1
S6K1	79	4
JNK2	80	2
DAPK1	81	7
MELK	82	5
PRK2	82	1
p38 delta MAPK	83	3
MNK1	83	11
MARK1	84	4
CSK	84	7
MAPKAP-K2	85	6
EPH-B3	85	1
CHK1	85	2
IRR	87	3
TAO1	88	3
Aurora B	88	4
CK1 delta	91	1
BRK	91	5
SGK1	93	5
MPSK1	93	11
CHK2	94	4
PAK5	95	0
MARK3	95	2
CK2	96	6
MNK2	96	5
ERK2	97	6
PDK1	98	3
p38 beta MAPK	99	2
MKK6	100	5
IGF-1R	101	1
EPH-A4	101	6
PHK	103	2
Lck	103	0
GSK3 beta	103	9
EPH-B1	104	6
EPH-B2	105	8
PKA	106	6
EF2K	106	1
PKC alpha	107	6
CDK2-Cyclin A	107	9
ERK1	108	4
ASK1	108	3
p38 gamma MAPK	109	9
EPH-B4	110	9
IR	110	5
ZAP70	111	4
MST4	129	6

Screen Conc: 1.00µM

Kinase	% Activity Remaining Sort descending	Standard Deviation
NEK2a	34	1
MINK1	50	3
Lck	52	9
VEGFR1	53	13
EPH-B3	57	0
NEK6	59	2
PKB beta	60	7
TIE2	60	10
IGF-1R	62	0
PKD1	64	4
PLK1	64	2
PKC alpha	65	13
Aurora B	67	3
YES1	68	1
MST4	69	2
FGF-R1	70	1
ERK8	70	7
RSK2	73	3
MKK1	74	0
TTK	76	1
PAK5	76	4
IKK beta	76	5
BTK	77	1
MLK1	78	15
PIM3	79	7
BRSK2	79	2
PIM1	80	15
p38 delta MAPK	80	2
JNK1	80	2
GSK3 beta	81	0
CAMK1	81	14
MLK3	81	4
GCK	81	1
MKK2	82	1
PRK2	83	0
DYRK1A	83	8
Src	83	7
CSK	83	8
ROCK 2	84	6
TBK1	84	11
DYRK3	84	5
ERK2	84	0
MAPKAP-K3	84	9
PRAK	85	10
NUAK1	85	16
PDK1	86	2
PAK6	86	6
PKC gamma	86	6
HIPK1	86	1
JNK3	87	0
PAK4	87	0
SYK	88	7
TAK1	88	9
MELK	88	9
PKB alpha	89	14
CK2	89	4
TAO1	89	5
SGK1	90	5
MARK3	90	6
Aurora A	90	0
IRR	91	8
CHK2	91	11
STK33	92	7
DYRK2	92	1
PKA	92	15
MARK2	93	11
PAK2	93	7
IRAK4	93	3
DAPK1	94	4
HIPK2	94	12
HIPK3	95	7
PIM2	95	6
MAPKAP-K2	95	4
SRPK1	95	1
HER4	95	1
BRK	95	8
PHK	95	3
MARK1	96	2
SmMLCK	96	1
EF2K	96	11
CHK1	96	5
AMPK	96	5
RIPK2	98	7
ERK1	98	11
RSK1	98	14
JAK2	99	8
MPSK1	99	14
MARK4	99	5
MST2	99	0
EPH-A2	99	3
ABL	100	6
PKC zeta	100	3
MNK2	101	9
CLK2	101	6
CK1 delta	102	13
JNK2	102	14
EPH-B1	103	3
CAMKK beta	103	4
p38 gamma MAPK	103	0
EPH-B4	104	5
S6K1	104	7
MNK1	104	2
EPH-B2	105	4
CDK2-Cyclin A	106	14
ZAP70	107	10
IR	107	9
IKK epsilon	108	5
p38 beta MAPK	108	3
EPH-A4	109	6
p38 alpha MAPK	110	6
ASK1	110	9
MSK1	110	1
BRSK1	111	11
MEKK1	113	0
LKB1	115	14
MKK6	123	7
TrkA	133	7

Screen Conc: 0.10µM

Kinase	% Activity Remaining Sort descending	Standard Deviation
CHK2	76	6
TAK1	76	3
MKK2	79	1
DAPK1	83	8
MINK1	84	8
PAK6	84	8
PRK2	86	4
STK33	86	8
IGF-1R	87	6
MKK1	87	0
p38 beta MAPK	88	6
EF2K	88	15
MLK1	89	2
Src	89	7
PAK2	90	12
MAPKAP-K2	91	12
NUAK1	93	6
PRAK	93	7
NEK2a	93	10
JAK2	93	0
IKK beta	94	2
AMPK	94	22
DYRK1A	94	1
PKB beta	94	19
MARK3	94	0
SGK1	94	8
p38 delta MAPK	94	9
RSK2	95	1
PKC alpha	95	5
PKC gamma	95	6
GSK3 beta	95	2
YES1	95	4
EPH-B3	95	16
NEK6	95	13
PKC zeta	96	5
SRPK1	96	0
PKD1	96	6
CDK2-Cyclin A	96	2
PAK4	96	2
MST4	97	6
MARK1	97	8
MEKK1	97	3
EPH-B1	97	11
PIM2	98	2
RIPK2	98	5
CK2	98	2
CAMK1	98	1
PIM1	98	3
ABL	98	11
IKK epsilon	99	3
MARK2	99	4
IRAK4	99	4
JNK1	100	7
MELK	100	33
EPH-B2	100	9
CSK	100	2
TTK	100	0
Aurora B	100	5
VEGFR1	101	19
MAPKAP-K3	101	15
TBK1	101	2
DYRK3	101	2
MLK3	101	7
HIPK1	102	1
HIPK3	102	9
PKB alpha	102	12
CLK2	102	9
MPSK1	102	10
Aurora A	102	3
TAO1	102	0
ROCK 2	103	3
CK1 delta	103	5
ASK1	104	5
p38 gamma MAPK	104	13
IRR	104	6
PAK5	104	6
ERK2	104	12
SmMLCK	104	4
RSK1	105	6
MNK1	105	2
JNK3	105	2
PIM3	105	10
MARK4	105	11
ERK1	105	1
ZAP70	106	48
HIPK2	106	6
PDK1	106	6
GCK	106	3
ERK8	106	10
EPH-B4	107	2
DYRK2	107	9
SYK	108	9
p38 alpha MAPK	108	7
TIE2	109	7
HER4	110	1
CHK1	110	2
BRSK1	110	5
S6K1	110	7
BRSK2	110	4
JNK2	111	10
MST2	112	32
EPH-A2	112	6
MKK6	112	1
MNK2	113	31
BRK	113	5
IR	113	5
EPH-A4	113	2
PKA	114	6
PLK1	115	4
Lck	116	11
MSK1	116	2
CAMKK beta	119	0
BTK	119	5
PHK	120	2
FGF-R1	120	4
LKB1	125	11
TrkA	144	33