Inhibitor | Chelerythrine Chloride  

Screen Conc: 0.10µM

Kinase	% Activity Remaining	Standard Deviation
ABL	98	11
AMPK	94	22
ASK1	104	5
Aurora A	102	3
Aurora B	100	5
BRK	113	5
BRSK1	110	5
BRSK2	110	4
BTK	119	5
CAMK1	98	1
CAMKK beta	119	0
CDK2-Cyclin A	96	2
CHK1	110	2
CHK2	76	6
CK1 delta	103	5
CK2	98	2
CLK2	102	9
CSK	100	2
DAPK1	83	8
DYRK1A	94	1
DYRK2	107	9
DYRK3	101	2
EF2K	88	15
EPH-A2	112	6
EPH-A4	113	2
EPH-B1	97	11
EPH-B2	100	9
EPH-B3	95	16
EPH-B4	107	2
ERK1	105	1
ERK2	104	12
ERK8	106	10
FGF-R1	120	4
GCK	106	3
GSK3 beta	95	2
HER4	110	1
HIPK1	102	1
HIPK2	106	6
HIPK3	102	9
IGF-1R	87	6
IKK beta	94	2
IKK epsilon	99	3
IR	113	5
IRAK4	99	4
IRR	104	6
JAK2	93	0
JNK1	100	7
JNK2	111	10
JNK3	105	2
Lck	116	11
LKB1	125	11
MAPKAP-K2	91	12
MAPKAP-K3	101	15
MARK1	97	8
MARK2	99	4
MARK3	94	0
MARK4	105	11
MEKK1	97	3
MELK	100	33
MINK1	84	8
MKK1	87	0
MKK2	79	1
MKK6	112	1
MLK1	89	2
MLK3	101	7
MNK1	105	2
MNK2	113	31
MPSK1	102	10
MSK1	116	2
MST2	112	32
MST4	97	6
NEK2a	93	10
NEK6	95	13
NUAK1	93	6
p38 alpha MAPK	108	7
p38 beta MAPK	88	6
p38 delta MAPK	94	9
p38 gamma MAPK	104	13
PAK2	90	12
PAK4	96	2
PAK5	104	6
PAK6	84	8
PDK1	106	6
PHK	120	2
PIM1	98	3
PIM2	98	2
PIM3	105	10
PKA	114	6
PKB alpha	102	12
PKB beta	94	19
PKC alpha	95	5
PKC gamma	95	6
PKC zeta	96	5
PKD1	96	6
PLK1	115	4
PRAK	93	7
PRK2	86	4
RIPK2	98	5
ROCK 2	103	3
RSK1	105	6
RSK2	95	1
S6K1	110	7
SGK1	94	8
SmMLCK	104	4
Src	89	7
SRPK1	96	0
STK33	86	8
SYK	108	9
TAK1	76	3
TAO1	102	0
TBK1	101	2
TIE2	109	7
TrkA	144	33
TTK	100	0
VEGFR1	101	19
YES1	95	4
ZAP70	106	48

Screen Conc: 1.00µM

Kinase	% Activity Remaining	Standard Deviation
ABL	100	6
AMPK	96	5
ASK1	110	9
Aurora A	90	0
Aurora B	67	3
BRK	95	8
BRSK1	111	11
BRSK2	79	2
BTK	77	1
CAMK1	81	14
CAMKK beta	103	4
CDK2-Cyclin A	106	14
CHK1	96	5
CHK2	91	11
CK1 delta	102	13
CK2	89	4
CLK2	101	6
CSK	83	8
DAPK1	94	4
DYRK1A	83	8
DYRK2	92	1
DYRK3	84	5
EF2K	96	11
EPH-A2	99	3
EPH-A4	109	6
EPH-B1	103	3
EPH-B2	105	4
EPH-B3	57	0
EPH-B4	104	5
ERK1	98	11
ERK2	84	0
ERK8	70	7
FGF-R1	70	1
GCK	81	1
GSK3 beta	81	0
HER4	95	1
HIPK1	86	1
HIPK2	94	12
HIPK3	95	7
IGF-1R	62	0
IKK beta	76	5
IKK epsilon	108	5
IR	107	9
IRAK4	93	3
IRR	91	8
JAK2	99	8
JNK1	80	2
JNK2	102	14
JNK3	87	0
Lck	52	9
LKB1	115	14
MAPKAP-K2	95	4
MAPKAP-K3	84	9
MARK1	96	2
MARK2	93	11
MARK3	90	6
MARK4	99	5
MEKK1	113	0
MELK	88	9
MINK1	50	3
MKK1	74	0
MKK2	82	1
MKK6	123	7
MLK1	78	15
MLK3	81	4
MNK1	104	2
MNK2	101	9
MPSK1	99	14
MSK1	110	1
MST2	99	0
MST4	69	2
NEK2a	34	1
NEK6	59	2
NUAK1	85	16
p38 alpha MAPK	110	6
p38 beta MAPK	108	3
p38 delta MAPK	80	2
p38 gamma MAPK	103	0
PAK2	93	7
PAK4	87	0
PAK5	76	4
PAK6	86	6
PDK1	86	2
PHK	95	3
PIM1	80	15
PIM2	95	6
PIM3	79	7
PKA	92	15
PKB alpha	89	14
PKB beta	60	7
PKC alpha	65	13
PKC gamma	86	6
PKC zeta	100	3
PKD1	64	4
PLK1	64	2
PRAK	85	10
PRK2	83	0
RIPK2	98	7
ROCK 2	84	6
RSK1	98	14
RSK2	73	3
S6K1	104	7
SGK1	90	5
SmMLCK	96	1
Src	83	7
SRPK1	95	1
STK33	92	7
SYK	88	7
TAK1	88	9
TAO1	89	5
TBK1	84	11
TIE2	60	10
TrkA	133	7
TTK	76	1
VEGFR1	53	13
YES1	68	1
ZAP70	107	10

Screen Conc: 10.00µM

Kinase	% Activity Remaining	Standard Deviation
ABL	78	6
AMPK	67	4
ASK1	108	3
Aurora A	62	6
Aurora B	88	4
BRK	91	5
BRSK1	41	9
BRSK2	68	8
BTK	36	2
CAMK1	12	3
CAMKK beta	61	3
CDK2-Cyclin A	107	9
CHK1	85	2
CHK2	94	4
CK1 delta	91	1
CK2	96	6
CLK2	66	1
CSK	84	7
DAPK1	81	7
DYRK1A	68	7
DYRK2	65	1
DYRK3	31	1
EF2K	106	1
EPH-A2	78	4
EPH-A4	101	6
EPH-B1	104	6
EPH-B2	105	8
EPH-B3	85	1
EPH-B4	110	9
ERK1	108	4
ERK2	97	6
ERK8	28	1
FGF-R1	29	3
GCK	27	2
GSK3 beta	103	9
HER4	61	3
HIPK1	58	1
HIPK2	32	8
HIPK3	22	1
IGF-1R	101	1
IKK beta	20	8
IKK epsilon	22	1
IR	110	5
IRAK4	27	5
IRR	87	3
JAK2	70	7
JNK1	57	1
JNK2	80	2
JNK3	61	6
Lck	103	0
LKB1	64	1
MAPKAP-K2	85	6
MAPKAP-K3	45	7
MARK1	84	4
MARK2	75	7
MARK3	95	2
MARK4	66	4
MEKK1	70	1
MELK	82	5
MINK1	6	0
MKK1	65	6
MKK2	72	1
MKK6	100	5
MLK1	13	1
MLK3	1	0
MNK1	83	11
MNK2	96	5
MPSK1	93	11
MSK1	74	1
MST2	75	0
MST4	129	6
NEK2a	18	0
NEK6	10	0
NUAK1	69	9
p38 alpha MAPK	71	1
p38 beta MAPK	99	2
p38 delta MAPK	83	3
p38 gamma MAPK	109	9
PAK2	79	7
PAK4	74	3
PAK5	95	0
PAK6	74	8
PDK1	98	3
PHK	103	2
PIM1	53	2
PIM2	75	5
PIM3	64	4
PKA	106	6
PKB alpha	75	2
PKB beta	22	2
PKC alpha	107	6
PKC gamma	51	2
PKC zeta	69	0
PKD1	67	2
PLK1	57	1
PRAK	69	0
PRK2	82	1
RIPK2	79	1
ROCK 2	74	0
RSK1	77	3
RSK2	47	6
S6K1	79	4
SGK1	93	5
SmMLCK	71	5
Src	40	7
SRPK1	71	2
STK33	79	1
SYK	48	2
TAK1	64	6
TAO1	88	3
TBK1	70	1
TIE2	18	1
TrkA	53	4
TTK	73	4
VEGFR1	10	3
YES1	45	8
ZAP70	111	4