Inhibitor | Withaferin A  

Screen Conc: 0.10µM

Kinase	% Activity Remaining	Standard Deviation
ABL	101	4
AMPK	99	1
AMPK (hum)	107	0
ASK1	104	12
Aurora A	108	2
Aurora B	108	9
BRK	109	5
BRSK1	106	3
BRSK2	99	9
BTK	118	3
CAMK1	92	13
CAMKK beta	115	11
CDK2-Cyclin A	108	6
CDK9-Cyclin T1	94	0
CHK1	109	9
CHK2	130	12
CK1 delta	92	3
CK1 gamma 2	109	3
CK2	79	6
CLK2	88	8
CSK	112	3
DAPK1	108	9
DDR2	105	5
DYRK1A	92	1
DYRK2	97	4
DYRK3	103	6
EF2K	104	5
EIF2AK3	129	15
EPH-A2	94	7
EPH-A4	107	12
EPH-B1	105	4
EPH-B2	116	1
EPH-B3	114	2
EPH-B4	120	12
ERK1	99	3
ERK2	100	29
ERK8	107	20
FGF-R1	98	0
GCK	98	3
GSK3 beta	114	10
HER4	113	13
HIPK1	110	9
HIPK2	108	8
HIPK3	96	14
IGF-1R	105	1
IKK beta	103	4
IKK epsilon	96	2
IR	113	22
IRAK1	101	2
IRAK4	97	23
IRR	99	11
JAK2	98	4
JNK1	103	0
JNK2	112	7
JNK3	122	11
Lck	104	26
LKB1	104	5
MAP4K3	81	14
MAP4K5	106	12
MAPKAP-K2	50	21
MAPKAP-K3	120	5
MARK1	106	3
MARK2	109	4
MARK3	99	3
MARK4	87	2
MEKK1	112	1
MELK	98	2
MINK1	94	5
MKK1	100	10
MKK2	108	11
MKK6	95	13
MLK1	91	13
MLK3	110	3
MNK1	98	3
MNK2	99	12
MPSK1	117	22
MSK1	104	17
MST2	123	2
MST3	111	4
MST4	97	0
NEK2a	93	2
NEK6	95	23
NUAK1	112	3
OSR1	128	0
p38 alpha MAPK	101	11
p38 beta MAPK	121	14
p38 delta MAPK	106	6
p38 gamma MAPK	114	6
PAK2	100	9
PAK4	94	11
PAK5	102	6
PAK6	125	3
PDGFRA	119	30
PDK1	107	8
PHK	110	4
PIM1	109	12
PIM2	96	0
PIM3	95	2
PKA	112	4
PKB alpha	118	14
PKB beta	92	16
PKC alpha	96	11
PKC gamma	119	18
PKC zeta	103	3
PKD1	109	14
PLK1	105	9
PRAK	105	21
PRK2	103	5
RIPK2	95	8
ROCK 2	103	6
RSK1	109	8
RSK2	114	18
S6K1	99	7
SGK1	110	8
SIK2	82	13
SIK3	105	15
SmMLCK	113	13
Src	114	7
SRPK1	89	7
STK33	116	1
SYK	155	61
TAK1	114	17
TAO1	103	14
TBK1	48	23
TESK1	109	2
TIE2	93	20
TLK1	101	7
TrkA	92	18
TSSK1	100	1
TTBK1	100	5
TTBK2	108	10
TTK	111	1
ULK1	108	17
ULK2	92	7
VEGFR1	120	18
WNK1	117	2
YES1	99	4
ZAP70	114	24

Screen Conc: 1.00µM

Kinase	% Activity Remaining	Standard Deviation
ABL	110	1
AMPK	100	5
AMPK (hum)	120	10
ASK1	100	13
Aurora A	102	6
Aurora B	86	7
BRK	123	7
BRSK1	111	2
BRSK2	103	2
BTK	115	12
CAMK1	84	5
CAMKK beta	115	15
CDK2-Cyclin A	113	18
CDK9-Cyclin T1	97	1
CHK1	114	9
CHK2	116	1
CK1 delta	103	9
CK1 gamma 2	104	10
CK2	94	8
CLK2	102	9
CSK	109	4
DAPK1	95	6
DDR2	100	6
DYRK1A	94	4
DYRK2	88	8
DYRK3	101	9
EF2K	100	4
EIF2AK3	108	4
EPH-A2	93	4
EPH-A4	98	6
EPH-B1	101	1
EPH-B2	102	1
EPH-B3	104	13
EPH-B4	119	6
ERK1	100	2
ERK2	98	24
ERK8	115	19
FGF-R1	97	2
GCK	106	4
GSK3 beta	124	5
HER4	109	3
HIPK1	112	8
HIPK2	113	12
HIPK3	94	3
IGF-1R	123	0
IKK beta	103	6
IKK epsilon	97	3
IR	117	12
IRAK1	99	11
IRAK4	101	7
IRR	97	1
JAK2	98	10
JNK1	102	4
JNK2	114	4
JNK3	125	3
Lck	111	15
LKB1	111	8
MAP4K3	85	5
MAP4K5	110	5
MAPKAP-K2	56	15
MAPKAP-K3	85	15
MARK1	115	8
MARK2	111	6
MARK3	114	17
MARK4	97	2
MEKK1	115	9
MELK	107	4
MINK1	88	8
MKK1	113	21
MKK2	126	27
MKK6	107	20
MLK1	100	5
MLK3	110	2
MNK1	100	6
MNK2	104	24
MPSK1	117	21
MSK1	101	3
MST2	126	3
MST3	126	10
MST4	95	15
NEK2a	94	1
NEK6	102	1
NUAK1	121	12
OSR1	109	6
p38 alpha MAPK	92	10
p38 beta MAPK	129	20
p38 delta MAPK	110	1
p38 gamma MAPK	116	3
PAK2	96	1
PAK4	104	6
PAK5	116	1
PAK6	126	2
PDGFRA	117	2
PDK1	104	2
PHK	112	3
PIM1	103	1
PIM2	102	10
PIM3	110	3
PKA	109	9
PKB alpha	99	0
PKB beta	72	8
PKC alpha	111	15
PKC gamma	112	1
PKC zeta	110	1
PKD1	103	11
PLK1	101	15
PRAK	108	30
PRK2	95	4
RIPK2	108	5
ROCK 2	107	8
RSK1	101	4
RSK2	98	4
S6K1	97	1
SGK1	110	20
SIK2	78	5
SIK3	106	3
SmMLCK	114	16
Src	113	8
SRPK1	86	1
STK33	112	22
SYK	115	6
TAK1	104	7
TAO1	113	3
TBK1	107	1
TESK1	114	9
TIE2	98	7
TLK1	103	2
TrkA	110	2
TSSK1	102	13
TTBK1	118	3
TTBK2	105	5
TTK	112	10
ULK1	115	7
ULK2	96	14
VEGFR1	91	20
WNK1	111	2
YES1	112	12
ZAP70	113	19

Screen Conc: 10.00µM

Kinase	% Activity Remaining	Standard Deviation
ABL	106	8
AMPK	90	2
AMPK (hum)	112	9
ASK1	118	21
Aurora A	30	2
Aurora B	10	1
BRK	115	20
BRSK1	91	4
BRSK2	77	0
BTK	110	9
CAMK1	30	1
CAMKK beta	99	16
CDK2-Cyclin A	117	7
CDK9-Cyclin T1	107	9
CHK1	117	7
CHK2	61	10
CK1 delta	105	5
CK1 gamma 2	103	7
CK2	94	1
CLK2	94	3
CSK	111	10
DAPK1	108	12
DDR2	94	1
DYRK1A	100	3
DYRK2	104	15
DYRK3	78	11
EF2K	97	9
EIF2AK3	115	1
EPH-A2	96	5
EPH-A4	109	4
EPH-B1	107	5
EPH-B2	105	10
EPH-B3	97	11
EPH-B4	105	2
ERK1	109	14
ERK2	111	9
ERK8	72	10
FGF-R1	94	6
GCK	68	9
GSK3 beta	127	12
HER4	101	0
HIPK1	101	2
HIPK2	60	2
HIPK3	58	7
IGF-1R	113	6
IKK beta	72	9
IKK epsilon	61	7
IR	128	33
IRAK1	100	1
IRAK4	79	7
IRR	77	2
JAK2	103	6
JNK1	110	0
JNK2	119	7
JNK3	136	3
Lck	129	15
LKB1	114	6
MAP4K3	84	0
MAP4K5	94	4
MAPKAP-K2	63	25
MAPKAP-K3	47	11
MARK1	111	3
MARK2	118	7
MARK3	118	7
MARK4	77	8
MEKK1	95	14
MELK	123	1
MINK1	81	2
MKK1	111	7
MKK2	120	2
MKK6	109	0
MLK1	86	17
MLK3	97	4
MNK1	108	1
MNK2	126	13
MPSK1	126	4
MSK1	99	10
MST2	129	1
MST3	133	8
MST4	80	21
NEK2a	82	4
NEK6	111	7
NUAK1	110	5
OSR1	125	10
p38 alpha MAPK	95	2
p38 beta MAPK	128	7
p38 delta MAPK	92	4
p38 gamma MAPK	103	2
PAK2	86	11
PAK4	104	11
PAK5	111	10
PAK6	105	5
PDGFRA	121	12
PDK1	116	14
PHK	105	5
PIM1	94	9
PIM2	97	6
PIM3	83	1
PKA	113	5
PKB alpha	105	3
PKB beta	10	1
PKC alpha	96	11
PKC gamma	102	4
PKC zeta	101	5
PKD1	104	17
PLK1	118	17
PRAK	105	24
PRK2	104	2
RIPK2	110	9
ROCK 2	112	2
RSK1	105	2
RSK2	74	12
S6K1	69	3
SGK1	103	19
SIK2	66	5
SIK3	94	8
SmMLCK	114	6
Src	93	21
SRPK1	63	8
STK33	120	11
SYK	130	15
TAK1	109	16
TAO1	120	10
TBK1	93	9
TESK1	104	6
TIE2	83	17
TLK1	98	13
TrkA	97	13
TSSK1	78	3
TTBK1	95	2
TTBK2	112	10
TTK	94	4
ULK1	111	0
ULK2	90	7
VEGFR1	30	8
WNK1	113	5
YES1	68	24
ZAP70	124	36