Inhibitor | OSU-03012  

Screen Conc: 1.00µM

Kinase	% Activity Remaining	Standard Deviation
ABL	95	11
AMPK	99	1
AMPK (hum)	88	1
ASK1	86	2
Aurora A	95	19
Aurora B	93	3
BRK	91	2
BRSK1	83	5
BRSK2	76	14
BTK	98	0
CAMK1	109	7
CAMKK beta	120	26
CDK2-Cyclin A	95	1
CDK9-Cyclin T1	82	0
CHK1	72	10
CHK2	98	22
CK1 delta	96	14
CK1 gamma 2	93	5
CK2	111	2
CLK2	93	6
CSK	97	12
DAPK1	95	4
DDR2	92	0
DYRK1A	105	27
DYRK2	89	1
DYRK3	108	6
EF2K	103	24
EIF2AK3	102	3
EPH-A2	92	9
EPH-A4	80	15
EPH-B1	72	3
EPH-B2	82	19
EPH-B3	72	2
EPH-B4	116	6
ERK1	80	11
ERK2	101	16
ERK5	91	2
ERK8	79	4
FGF-R1	111	17
GCK	107	11
GSK3 beta	110	2
HER4	106	11
HIPK1	104	4
HIPK2	88	5
HIPK3	94	3
IGF-1R	100	23
IKK beta	87	3
IKK epsilon	114	33
IR	98	0
IRAK1	98	20
IRAK4	84	7
IRR	91	3
JAK2	101	0
JNK1	107	11
JNK2	102	8
JNK3	85	2
Lck	84	3
LKB1	103	3
MAP4K3	84	14
MAP4K5	80	1
MAPKAP-K2	91	1
MAPKAP-K3	103	6
MARK1	87	2
MARK2	94	7
MARK3	96	3
MARK4	88	5
MEKK1	93	6
MELK	92	2
MINK1	78	6
MKK1	108	1
MKK2	123	10
MKK6	82	4
MLK1	99	10
MLK3	94	11
MNK1	86	2
MNK2	91	11
MPSK1	100	24
MSK1	110	10
MST2	106	18
MST3	84	8
MST4	80	8
NEK2a	96	9
NEK6	115	7
NUAK1	85	4
OSR1	95	4
p38 alpha MAPK	100	0
p38 beta MAPK	92	10
p38 delta MAPK	96	11
p38 gamma MAPK	94	7
PAK2	107	6
PAK4	84	1
PAK5	87	7
PAK6	88	3
PDGFRA	82	1
PDK1	79	6
PHK	79	15
PIM1	92	8
PIM2	93	4
PIM3	99	11
PKA	100	12
PKB alpha	91	16
PKB beta	87	8
PKC alpha	94	1
PKC gamma	95	3
PKC zeta	94	3
PKD1	86	9
PLK1	95	6
PRAK	89	6
PRK2	85	1
RIPK2	93	9
ROCK 2	88	7
RSK1	87	9
RSK2	80	23
S6K1	86	13
SGK1	71	2
SIK2	95	6
SIK3	91	4
SmMLCK	109	6
Src	103	18
SRPK1	94	2
STK33	91	2
SYK	87	21
TAK1	74	7
TAO1	103	5
TBK1	120	31
TESK1	84	7
TGFBR1	98	7
TIE2	103	13
TLK1	89	3
TrkA	90	5
TSSK1	102	20
TTBK1	88	6
TTBK2	96	8
TTK	90	9
ULK1	86	3
ULK2	83	21
VEGFR1	79	16
WNK1	119	21
YES1	85	13
ZAP70	84	3

Screen Conc: 10.00µM

Kinase	% Activity Remaining	Standard Deviation
ABL	103	6
AMPK	105	10
AMPK (hum)	94	11
ASK1	83	0
Aurora A	107	13
Aurora B	88	3
BRK	91	3
BRSK1	89	6
BRSK2	64	3
BTK	96	11
CAMK1	21	0
CAMKK beta	115	4
CDK2-Cyclin A	95	2
CDK9-Cyclin T1	83	3
CHK1	44	4
CHK2	76	14
CK1 delta	93	12
CK1 gamma 2	90	7
CK2	79	11
CLK2	64	7
CSK	85	13
DAPK1	43	8
DDR2	101	8
DYRK1A	82	18
DYRK2	121	4
DYRK3	105	21
EF2K	75	18
EIF2AK3	95	20
EPH-A2	94	2
EPH-A4	91	2
EPH-B1	67	9
EPH-B2	91	4
EPH-B3	82	9
EPH-B4	88	21
ERK1	63	1
ERK2	88	13
ERK5	89	1
ERK8	75	4
FGF-R1	128	20
GCK	92	9
GSK3 beta	119	4
HER4	104	7
HIPK1	90	19
HIPK2	69	14
HIPK3	64	3
IGF-1R	105	4
IKK beta	88	3
IKK epsilon	101	2
IR	94	2
IRAK1	80	11
IRAK4	79	8
IRR	75	12
JAK2	116	27
JNK1	103	3
JNK2	96	8
JNK3	86	10
Lck	247	13
LKB1	99	3
MAP4K3	73	19
MAP4K5	73	0
MAPKAP-K2	95	2
MAPKAP-K3	97	1
MARK1	79	3
MARK2	89	7
MARK3	101	2
MARK4	102	9
MEKK1	103	3
MELK	75	5
MINK1	47	7
MKK1	107	7
MKK2	113	14
MKK6	74	4
MLK1	80	2
MLK3	87	9
MNK1	83	0
MNK2	109	17
MPSK1	97	20
MSK1	46	3
MST2	81	15
MST3	70	6
MST4	72	3
NEK2a	92	8
NEK6	63	0
NUAK1	61	4
OSR1	106	26
p38 alpha MAPK	80	4
p38 beta MAPK	95	7
p38 delta MAPK	70	2
p38 gamma MAPK	70	1
PAK2	75	7
PAK4	70	1
PAK5	107	9
PAK6	28	1
PDGFRA	98	8
PDK1	77	7
PHK	72	0
PIM1	94	2
PIM2	98	4
PIM3	64	2
PKA	93	10
PKB alpha	53	0
PKB beta	54	2
PKC alpha	90	9
PKC gamma	97	0
PKC zeta	61	3
PKD1	93	10
PLK1	111	11
PRAK	76	11
PRK2	63	3
RIPK2	81	13
ROCK 2	87	8
RSK1	63	9
RSK2	98	60
S6K1	21	7
SGK1	30	1
SIK2	79	3
SIK3	101	1
SmMLCK	42	30
Src	135	15
SRPK1	80	3
STK33	92	9
SYK	85	2
TAK1	36	11
TAO1	92	7
TBK1	83	7
TESK1	103	6
TGFBR1	98	0
TIE2	105	24
TLK1	104	14
TrkA	90	3
TSSK1	83	15
TTBK1	129	28
TTBK2	154	2
TTK	86	1
ULK1	97	14
ULK2	101	22
VEGFR1	89	9
WNK1	105	1
YES1	80	2
ZAP70	83	19