Inhibitor | PF3644022  

Screen Conc: 10.00µM

Kinase	% Activity Remaining Sort descending	Standard Deviation
MAPKAP-K2	2	1
PIM3	3	2
AMPK (hum)	6	0
DYRK1A	8	0
BRSK2	8	2
CLK2	9	2
CHK2	9	8
AMPK	9	0
ASK1	10	1
DAPK1	15	2
PIM1	17	3
GCK	19	0
SRPK1	19	2
IRAK4	24	5
PRAK	24	7
MNK2	26	7
IRAK1	27	2
CAMKK beta	28	3
BRSK1	29	0
MAPKAP-K3	30	10
STK33	38	12
ERK8	38	5
RIPK2	39	18
DYRK3	40	4
MINK1	40	0
MAP4K3	41	0
IKK epsilon	44	2
PKD1	48	7
MARK4	49	11
RSK2	50	10
CDK9-Cyclin T1	51	13
DYRK2	52	1
IRR	53	16
ULK2	54	4
YES1	55	3
CDK2-Cyclin A	57	9
NUAK1	58	4
MLK1	60	30
MAP4K5	61	5
Aurora B	64	8
VEGFR1	65	7
PIM2	66	9
NEK6	66	3
CAMK1	68	14
FGF-R1	68	14
MKK1	69	4
PKB beta	69	1
TAO1	70	2
TAK1	70	7
SIK3	73	14
EIF2AK3	73	0
MLK3	73	1
ULK1	75	4
EPH-A2	76	5
ABL	76	6
RSK1	76	2
SIK2	77	17
MELK	77	7
EPH-A4	78	1
HIPK2	78	4
JAK2	79	1
MKK2	79	4
S6K1	80	2
ERK2	81	16
PRK2	81	0
PAK4	82	11
SYK	82	10
MNK1	83	12
TrkA	83	4
p38 alpha MAPK	83	2
TTK	84	12
Aurora A	84	4
EF2K	85	1
EPH-B3	85	2
PHK	86	5
Lck	86	20
NEK2a	88	14
GSK3 beta	88	4
TIE2	88	7
TSSK1	89	1
PKC alpha	90	6
MST4	91	1
DDR2	91	8
MKK6	91	0
BRK	92	9
JNK1	92	2
CK1 delta	92	8
MARK1	93	2
PAK2	93	10
CK2	93	3
p38 delta MAPK	94	8
MARK2	95	1
ROCK 2	95	2
PDK1	95	12
ZAP70	95	21
HIPK3	96	14
TTBK1	96	14
TBK1	96	10
LKB1	97	7
p38 gamma MAPK	97	7
PLK1	98	8
PKB alpha	98	4
HER4	99	2
MSK1	99	5
EPH-B4	99	3
TLK1	100	6
Src	100	10
MST2	100	9
TESK1	101	5
PAK5	101	2
PKC gamma	101	5
IKK beta	101	1
CK1 gamma 2	102	3
JNK3	102	16
TTBK2	103	15
PDGFRA	104	51
MPSK1	105	13
CSK	106	18
EPH-B1	106	2
PKC zeta	106	8
BTK	107	4
JNK2	107	9
PKA	108	6
MST3	108	21
MEKK1	109	6
SmMLCK	110	7
MARK3	110	28
EPH-B2	111	0
ERK1	112	20
CHK1	112	6
SGK1	112	17
IGF-1R	114	3
PAK6	116	7
p38 beta MAPK	117	10
OSR1	118	5
WNK1	119	3
HIPK1	130	8
IR	133	40

Screen Conc: 1.00µM

Kinase	% Activity Remaining Sort descending	Standard Deviation
MAPKAP-K2	9	3
PIM3	18	3
AMPK (hum)	21	0
DYRK1A	31	3
BRSK2	32	6
AMPK	38	1
CLK2	43	2
CHK2	51	2
MNK2	51	20
BRSK1	55	1
PIM1	58	1
DAPK1	62	0
IRAK4	62	2
MAPKAP-K3	62	19
SRPK1	63	0
PRAK	67	11
MAP4K3	72	12
IRAK1	77	11
ERK8	79	10
IRR	79	10
RSK2	79	11
ASK1	80	5
STK33	83	4
ERK2	84	25
DYRK3	84	23
TSSK1	85	8
SIK2	86	7
TIE2	86	1
MARK4	87	3
TBK1	87	5
CAMKK beta	87	23
PAK4	88	4
EPH-B3	88	18
GCK	89	1
ULK1	89	14
YES1	89	1
PKB beta	90	18
PHK	91	5
FGF-R1	91	11
JNK1	93	2
CDK2-Cyclin A	93	12
RIPK2	94	24
p38 alpha MAPK	94	7
BRK	94	9
IKK epsilon	95	12
MLK1	95	13
Lck	95	8
EPH-A2	95	6
S6K1	95	0
ROCK 2	96	1
NEK6	96	1
PKD1	96	6
CDK9-Cyclin T1	96	4
CK1 delta	96	5
MKK1	97	15
PKB alpha	97	13
EF2K	97	10
MINK1	97	9
CK2	97	4
CAMK1	97	12
TAO1	98	4
SYK	98	25
MKK6	98	22
NEK2a	99	17
GSK3 beta	99	1
SIK3	99	15
Aurora B	99	7
RSK1	100	2
TrkA	100	0
ZAP70	100	13
PRK2	101	9
PAK5	101	10
DDR2	101	8
MST4	101	1
TTK	101	0
EIF2AK3	101	2
JAK2	102	6
MELK	102	20
NUAK1	102	5
Aurora A	102	14
MLK3	102	7
PAK2	103	11
PIM2	103	11
MNK1	103	3
LKB1	103	8
p38 delta MAPK	103	8
EPH-A4	103	9
JNK3	104	3
TTBK1	104	14
HIPK1	104	1
DYRK2	105	6
MSK1	105	2
HER4	105	10
EPH-B1	106	1
PAK6	106	8
EPH-B4	106	9
PDK1	107	3
TLK1	107	3
MARK1	107	2
WNK1	108	0
PDGFRA	108	12
MKK2	108	6
MAP4K5	108	15
IGF-1R	108	6
ERK1	108	3
HIPK3	108	6
TAK1	109	9
CHK1	109	10
IKK beta	109	0
MST3	109	4
MEKK1	109	0
EPH-B2	110	15
PKC gamma	110	9
JNK2	111	6
CSK	111	21
TESK1	111	7
SmMLCK	112	3
PKC zeta	112	13
OSR1	112	2
CK1 gamma 2	112	2
SGK1	112	7
MARK2	113	3
ABL	113	6
PKC alpha	113	22
HIPK2	113	1
PLK1	114	8
MARK3	115	0
p38 gamma MAPK	115	14
Src	116	5
BTK	118	11
VEGFR1	118	35
MPSK1	118	3
PKA	119	2
ULK2	121	50
TTBK2	122	18
IR	125	27
p38 beta MAPK	126	3
MST2	135	34

Screen Conc: 0.10µM

Kinase	% Activity Remaining Sort descending	Standard Deviation
MAPKAP-K2	24	6
PIM3	55	3
AMPK (hum)	68	1
BRSK2	77	13
DYRK1A	80	4
BRSK1	86	7
CLK2	86	6
SRPK1	89	2
EPH-A2	90	3
HER4	92	1
ZAP70	93	9
AMPK	93	5
RIPK2	93	8
PIM1	93	9
SYK	94	6
p38 alpha MAPK	94	2
IRAK4	94	6
FGF-R1	95	3
MKK6	95	8
CK1 delta	96	12
PKD1	96	6
CAMK1	96	3
ERK8	96	3
JNK1	96	10
TSSK1	97	6
EPH-B3	98	12
MINK1	98	20
IKK epsilon	98	6
SIK3	98	13
MAP4K3	98	3
MLK1	99	4
S6K1	99	3
SIK2	99	6
MST4	99	23
EPH-A4	99	12
IRR	99	6
EPH-B2	99	8
CK2	100	5
PKB beta	100	24
PKC alpha	101	8
MKK1	101	12
IGF-1R	101	10
DYRK2	101	3
JAK2	101	4
MEKK1	102	16
ERK2	102	9
PLK1	102	7
PAK2	102	19
TTBK1	103	17
PIM2	103	15
DAPK1	103	10
PAK4	103	7
PDK1	104	13
IRAK1	104	16
PDGFRA	104	3
RSK2	104	18
DDR2	105	1
TIE2	105	14
STK33	105	1
MNK1	105	6
EF2K	106	1
RSK1	106	1
ASK1	106	18
CAMKK beta	107	0
MARK4	107	8
PAK5	108	6
Lck	108	20
p38 gamma MAPK	108	1
p38 delta MAPK	108	6
PRK2	108	12
NEK2a	108	17
NEK6	109	11
ULK2	109	17
PKB alpha	109	11
CDK9-Cyclin T1	109	3
PKA	109	7
GCK	109	11
TAO1	109	2
Aurora B	109	2
EPH-B4	110	16
Src	110	6
TBK1	110	9
PHK	110	12
HIPK1	111	1
CDK2-Cyclin A	111	1
ERK1	111	7
DYRK3	111	0
MNK2	111	4
MAP4K5	111	18
CHK2	112	16
VEGFR1	112	20
NUAK1	112	20
HIPK3	112	4
TESK1	112	9
YES1	112	3
ROCK 2	112	6
TLK1	113	1
MKK2	113	7
OSR1	113	13
MARK3	113	3
WNK1	113	2
EPH-B1	113	2
TAK1	113	9
MAPKAP-K3	114	5
EIF2AK3	114	10
MLK3	114	1
IKK beta	115	0
PKC zeta	115	6
ULK1	115	3
SGK1	116	19
MARK1	116	8
MARK2	116	2
PAK6	117	1
CSK	117	22
TTK	117	8
MPSK1	117	13
PKC gamma	118	7
LKB1	118	6
p38 beta MAPK	118	8
MST3	118	26
SmMLCK	118	4
JNK3	120	8
MSK1	120	3
ABL	120	3
BTK	120	11
TrkA	121	22
BRK	121	18
TTBK2	122	14
MELK	123	3
HIPK2	123	29
IR	123	17
JNK2	123	6
CK1 gamma 2	123	4
PRAK	126	20
GSK3 beta	126	2
Aurora A	126	20
MST2	131	16
CHK1	139	34