Inhibitor | PF3644022  

Screen Conc: 0.10µM

Kinase	% Activity Remaining	Standard Deviation
ABL	120	3
AMPK	93	5
AMPK (hum)	68	1
ASK1	106	18
Aurora A	126	20
Aurora B	109	2
BRK	121	18
BRSK1	86	7
BRSK2	77	13
BTK	120	11
CAMK1	96	3
CAMKK beta	107	0
CDK2-Cyclin A	111	1
CDK9-Cyclin T1	109	3
CHK1	139	34
CHK2	112	16
CK1 delta	96	12
CK1 gamma 2	123	4
CK2	100	5
CLK2	86	6
CSK	117	22
DAPK1	103	10
DDR2	105	1
DYRK1A	80	4
DYRK2	101	3
DYRK3	111	0
EF2K	106	1
EIF2AK3	114	10
EPH-A2	90	3
EPH-A4	99	12
EPH-B1	113	2
EPH-B2	99	8
EPH-B3	98	12
EPH-B4	110	16
ERK1	111	7
ERK2	102	9
ERK8	96	3
FGF-R1	95	3
GCK	109	11
GSK3 beta	126	2
HER4	92	1
HIPK1	111	1
HIPK2	123	29
HIPK3	112	4
IGF-1R	101	10
IKK beta	115	0
IKK epsilon	98	6
IR	123	17
IRAK1	104	16
IRAK4	94	6
IRR	99	6
JAK2	101	4
JNK1	96	10
JNK2	123	6
JNK3	120	8
Lck	108	20
LKB1	118	6
MAP4K3	98	3
MAP4K5	111	18
MAPKAP-K2	24	6
MAPKAP-K3	114	5
MARK1	116	8
MARK2	116	2
MARK3	113	3
MARK4	107	8
MEKK1	102	16
MELK	123	3
MINK1	98	20
MKK1	101	12
MKK2	113	7
MKK6	95	8
MLK1	99	4
MLK3	114	1
MNK1	105	6
MNK2	111	4
MPSK1	117	13
MSK1	120	3
MST2	131	16
MST3	118	26
MST4	99	23
NEK2a	108	17
NEK6	109	11
NUAK1	112	20
OSR1	113	13
p38 alpha MAPK	94	2
p38 beta MAPK	118	8
p38 delta MAPK	108	6
p38 gamma MAPK	108	1
PAK2	102	19
PAK4	103	7
PAK5	108	6
PAK6	117	1
PDGFRA	104	3
PDK1	104	13
PHK	110	12
PIM1	93	9
PIM2	103	15
PIM3	55	3
PKA	109	7
PKB alpha	109	11
PKB beta	100	24
PKC alpha	101	8
PKC gamma	118	7
PKC zeta	115	6
PKD1	96	6
PLK1	102	7
PRAK	126	20
PRK2	108	12
RIPK2	93	8
ROCK 2	112	6
RSK1	106	1
RSK2	104	18
S6K1	99	3
SGK1	116	19
SIK2	99	6
SIK3	98	13
SmMLCK	118	4
Src	110	6
SRPK1	89	2
STK33	105	1
SYK	94	6
TAK1	113	9
TAO1	109	2
TBK1	110	9
TESK1	112	9
TIE2	105	14
TLK1	113	1
TrkA	121	22
TSSK1	97	6
TTBK1	103	17
TTBK2	122	14
TTK	117	8
ULK1	115	3
ULK2	109	17
VEGFR1	112	20
WNK1	113	2
YES1	112	3
ZAP70	93	9

Screen Conc: 1.00µM

Kinase	% Activity Remaining	Standard Deviation
ABL	113	6
AMPK	38	1
AMPK (hum)	21	0
ASK1	80	5
Aurora A	102	14
Aurora B	99	7
BRK	94	9
BRSK1	55	1
BRSK2	32	6
BTK	118	11
CAMK1	97	12
CAMKK beta	87	23
CDK2-Cyclin A	93	12
CDK9-Cyclin T1	96	4
CHK1	109	10
CHK2	51	2
CK1 delta	96	5
CK1 gamma 2	112	2
CK2	97	4
CLK2	43	2
CSK	111	21
DAPK1	62	0
DDR2	101	8
DYRK1A	31	3
DYRK2	105	6
DYRK3	84	23
EF2K	97	10
EIF2AK3	101	2
EPH-A2	95	6
EPH-A4	103	9
EPH-B1	106	1
EPH-B2	110	15
EPH-B3	88	18
EPH-B4	106	9
ERK1	108	3
ERK2	84	25
ERK8	79	10
FGF-R1	91	11
GCK	89	1
GSK3 beta	99	1
HER4	105	10
HIPK1	104	1
HIPK2	113	1
HIPK3	108	6
IGF-1R	108	6
IKK beta	109	0
IKK epsilon	95	12
IR	125	27
IRAK1	77	11
IRAK4	62	2
IRR	79	10
JAK2	102	6
JNK1	93	2
JNK2	111	6
JNK3	104	3
Lck	95	8
LKB1	103	8
MAP4K3	72	12
MAP4K5	108	15
MAPKAP-K2	9	3
MAPKAP-K3	62	19
MARK1	107	2
MARK2	113	3
MARK3	115	0
MARK4	87	3
MEKK1	109	0
MELK	102	20
MINK1	97	9
MKK1	97	15
MKK2	108	6
MKK6	98	22
MLK1	95	13
MLK3	102	7
MNK1	103	3
MNK2	51	20
MPSK1	118	3
MSK1	105	2
MST2	135	34
MST3	109	4
MST4	101	1
NEK2a	99	17
NEK6	96	1
NUAK1	102	5
OSR1	112	2
p38 alpha MAPK	94	7
p38 beta MAPK	126	3
p38 delta MAPK	103	8
p38 gamma MAPK	115	14
PAK2	103	11
PAK4	88	4
PAK5	101	10
PAK6	106	8
PDGFRA	108	12
PDK1	107	3
PHK	91	5
PIM1	58	1
PIM2	103	11
PIM3	18	3
PKA	119	2
PKB alpha	97	13
PKB beta	90	18
PKC alpha	113	22
PKC gamma	110	9
PKC zeta	112	13
PKD1	96	6
PLK1	114	8
PRAK	67	11
PRK2	101	9
RIPK2	94	24
ROCK 2	96	1
RSK1	100	2
RSK2	79	11
S6K1	95	0
SGK1	112	7
SIK2	86	7
SIK3	99	15
SmMLCK	112	3
Src	116	5
SRPK1	63	0
STK33	83	4
SYK	98	25
TAK1	109	9
TAO1	98	4
TBK1	87	5
TESK1	111	7
TIE2	86	1
TLK1	107	3
TrkA	100	0
TSSK1	85	8
TTBK1	104	14
TTBK2	122	18
TTK	101	0
ULK1	89	14
ULK2	121	50
VEGFR1	118	35
WNK1	108	0
YES1	89	1
ZAP70	100	13

Screen Conc: 10.00µM

Kinase	% Activity Remaining	Standard Deviation
ABL	76	6
AMPK	9	0
AMPK (hum)	6	0
ASK1	10	1
Aurora A	84	4
Aurora B	64	8
BRK	92	9
BRSK1	29	0
BRSK2	8	2
BTK	107	4
CAMK1	68	14
CAMKK beta	28	3
CDK2-Cyclin A	57	9
CDK9-Cyclin T1	51	13
CHK1	112	6
CHK2	9	8
CK1 delta	92	8
CK1 gamma 2	102	3
CK2	93	3
CLK2	9	2
CSK	106	18
DAPK1	15	2
DDR2	91	8
DYRK1A	8	0
DYRK2	52	1
DYRK3	40	4
EF2K	85	1
EIF2AK3	73	0
EPH-A2	76	5
EPH-A4	78	1
EPH-B1	106	2
EPH-B2	111	0
EPH-B3	85	2
EPH-B4	99	3
ERK1	112	20
ERK2	81	16
ERK8	38	5
FGF-R1	68	14
GCK	19	0
GSK3 beta	88	4
HER4	99	2
HIPK1	130	8
HIPK2	78	4
HIPK3	96	14
IGF-1R	114	3
IKK beta	101	1
IKK epsilon	44	2
IR	133	40
IRAK1	27	2
IRAK4	24	5
IRR	53	16
JAK2	79	1
JNK1	92	2
JNK2	107	9
JNK3	102	16
Lck	86	20
LKB1	97	7
MAP4K3	41	0
MAP4K5	61	5
MAPKAP-K2	2	1
MAPKAP-K3	30	10
MARK1	93	2
MARK2	95	1
MARK3	110	28
MARK4	49	11
MEKK1	109	6
MELK	77	7
MINK1	40	0
MKK1	69	4
MKK2	79	4
MKK6	91	0
MLK1	60	30
MLK3	73	1
MNK1	83	12
MNK2	26	7
MPSK1	105	13
MSK1	99	5
MST2	100	9
MST3	108	21
MST4	91	1
NEK2a	88	14
NEK6	66	3
NUAK1	58	4
OSR1	118	5
p38 alpha MAPK	83	2
p38 beta MAPK	117	10
p38 delta MAPK	94	8
p38 gamma MAPK	97	7
PAK2	93	10
PAK4	82	11
PAK5	101	2
PAK6	116	7
PDGFRA	104	51
PDK1	95	12
PHK	86	5
PIM1	17	3
PIM2	66	9
PIM3	3	2
PKA	108	6
PKB alpha	98	4
PKB beta	69	1
PKC alpha	90	6
PKC gamma	101	5
PKC zeta	106	8
PKD1	48	7
PLK1	98	8
PRAK	24	7
PRK2	81	0
RIPK2	39	18
ROCK 2	95	2
RSK1	76	2
RSK2	50	10
S6K1	80	2
SGK1	112	17
SIK2	77	17
SIK3	73	14
SmMLCK	110	7
Src	100	10
SRPK1	19	2
STK33	38	12
SYK	82	10
TAK1	70	7
TAO1	70	2
TBK1	96	10
TESK1	101	5
TIE2	88	7
TLK1	100	6
TrkA	83	4
TSSK1	89	1
TTBK1	96	14
TTBK2	103	15
TTK	84	12
ULK1	75	4
ULK2	54	4
VEGFR1	65	7
WNK1	119	3
YES1	55	3
ZAP70	95	21