Inhibitor | BIO (6-Bromoindirubin-3'-oxime)  

Compound name Brutto MW CAS PubChem CID Action Reference Reference
BIO (6-Bromoindirubin-3'-oxime)   C16H10BrN3O2 356.20 667463-62-9 5287844 GSK3 Tseng A.S. (2006) Chem. Biol. 13 957 Polychronopoulos P. (2004) J. Med. Chem. 47 935

Screening Results Chart

Screening Results Tables

Screen Conc: 0.01µM
Kinase % Activity Remaining Standard Deviation
AMPK 91 21
Aurora B 94 11
BRSK2 112 5
BTK 93 12
CAMK1 97 17
CAMKK beta 93 0
CDK2-Cyclin A 88 7
CHK1 82 18
CHK2 77 19
CK1 delta 87 10
CK2 96 3
CSK 109 9
DYRK1A 102 10
DYRK2 93 16
DYRK3 108 4
EF2K 108 9
EPH-A2 94 3
EPH-B3 89 10
ERK1 88 13
ERK2 93 12
ERK8 95 10
FGF-R1 95 5
GSK3 beta 85 9
HIPK2 101 2
IGF-1R 106 8
IKK beta 106 11
IKK epsilon 101 0
IR 107 0
IRR 93 8
JNK1 98 6
JNK2 94 5
Lck 89 10
MAPKAP-K2 103 6
MARK3 97 4
MELK 86 12
MKK1 96 1
MNK1 118 4
MNK2 86 5
MSK1 97 6
MST2 87 8
MST4 98 4
NEK2a 96 6
NEK6 88 12
p38 alpha MAPK 103 22
p38 beta MAPK 87 7
p38 delta MAPK 108 10
p38 gamma MAPK 95 1
PAK4 95 18
PAK5 107 0
PAK6 102 16
PDK1 100 6
PHK 101 18
PIM1 100 10
PIM2 111 5
PIM3 96 8
PKA 102 1
PKB alpha 89 10
PKB beta 100 3
PKC alpha 113 1
PKC zeta 107 12
PKD1 98 6
PLK1 91 6
PRAK 97 3
PRK2 103 15
ROCK 2 100 5
RSK1 107 3
RSK2 93 4
S6K1 87 10
SGK1 88 8
SmMLCK 84 19
Src 73 13
SRPK1 87 8
SYK 99 2
TBK1 94 13
VEGFR1 95 6
YES1 76 6
Screen Conc: 0.10µM
Kinase % Activity Remaining Standard Deviation
AMPK 40 5
ASK1 94 2
Aurora A 50 3
Aurora B 51 11
BRSK1 14 3
BRSK2 48 1
BTK 29 4
CAMK1 26 7
CAMKK beta 83 2
CDK2-Cyclin A 29 1
CHK1 78 5
CHK2 67 3
CK1 delta 111 4
CK2 92 1
CLK2 81 1
CSK 87 8
DAPK1 60 0
DYRK1A 91 5
DYRK2 85 8
DYRK3 96 2
EF2K 85 2
EPH-A2 75 14
EPH-A4 88 5
EPH-B1 102 1
EPH-B2 85 6
EPH-B3 93 5
EPH-B4 111 3
ERK1 104 3
ERK2 112 12
ERK8 76 2
FGF-R1 43 5
GCK 52 0
GSK3 beta
7
 0
HER4 100 1
HIPK1 98 3
HIPK2 58 2
HIPK3 108 14
IGF-1R 63 5
IKK beta 106 5
IKK epsilon 80 12
IR 129 12
IRAK4 74 8
IRR 88 2
JAK2 56 2
JNK1 110 2
JNK2 105 9
JNK3 88 6
Lck 38 2
LKB1 75 1
MAPKAP-K2 91 2
MAPKAP-K3 91 11
MARK1 100 8
MARK2 94 5
MARK3 72 5
MARK4 81 4
MEKK1 78 7
MELK 71 1
MINK1 67 1
MKK1 100 13
MKK2 104 9
MKK6 118 12
MLK1 48 2
MLK3 14 3
MNK1 96 1
MNK2 76 2
MSK1 94 8
MST2 19 1
MST4 112 1
NEK2a 102 3
NEK6 102 8
NUAK1 51 8
p38 alpha MAPK 105 2
p38 beta MAPK 77 6
p38 delta MAPK 106 4
p38 gamma MAPK 94 5
PAK2 77 1
PAK4 78 7
PAK5 92 2
PAK6 100 6
PDK1 84 6
PHK 51 3
PIM1 99 7
PIM2 88 8
PIM3 73 3
PKA 109 4
PKB alpha 104 2
PKB beta 86 1
PKC alpha 97 5
PKC zeta 96 1
PKD1 112 15
PLK1 95 6
PRAK 103 2
PRK2 103 2
RIPK2 111 6
ROCK 2 105 8
RSK1 82 2
RSK2 114 6
S6K1 93 6
SGK1 77 10
SmMLCK 71 6
Src 16 5
SRPK1 107 10
SYK 75 9
TAK1 71 2
TAO1 109 7
TBK1 54 0
TrkA 13 0
TTK 86 6
VEGFR1 27 1
YES1 41 4
Screen Conc: 1.00µM
Kinase % Activity Remaining Standard Deviation
AMPK
6
 0
BRSK2 13 4
BTK
2
 0
CAMK1
10
 0
CAMKK beta
9
 0
CDK2-Cyclin A
4
 0
CHK1 12 3
CHK2
4
 2
CK1 delta 57 3
CK2 76 0
CSK 20 0
DYRK1A 22 0
DYRK2 44 2
DYRK3 58 0
EF2K 81 2
EPH-A2 11 0
EPH-B3 35 13
ERK1 67 4
ERK2 88 2
ERK8
2
 0
FGF-R1
3
 0
GSK3 beta
8
 8
HIPK2
3
 0
IGF-1R 11 2
IKK beta 86 6
IKK epsilon
9
 0
IR 38 3
IRR 18 3
JNK1 57 4
JNK2 53 1
Lck
3
 1
MAPKAP-K2 68 7
MARK3 17 4
MELK
4
 2
MKK1 35 4
MNK1 55 3
MNK2 50 0
MSK1 27 2
MST2
2
 0
MST4 11 0
NEK2a 91 16
NEK6 73 5
p38 alpha MAPK 70 19
p38 beta MAPK 41 0
p38 delta MAPK 70 0
p38 gamma MAPK 49 2
PAK4
9
 4
PAK5 13 0
PAK6 22 0
PDK1
1
 0
PHK
3
 0
PIM1 35 2
PIM2 26 9
PIM3 12 3
PKA 81 4
PKB alpha 72 3
PKB beta 51 6
PKC alpha 37 0
PKC zeta 69 4
PKD1 66 3
PLK1 51 8
PRAK 45 5
PRK2 45 7
ROCK 2 24 1
RSK1
7
 1
RSK2 35 3
S6K1 21 1
SGK1
9
 0
SmMLCK 14 3
Src
5
 1
SRPK1 68 5
SYK 17 20
TBK1
7
 1
VEGFR1
5
 2
YES1
4
 0
Screen Conc: 10.00µM
Kinase % Activity Remaining Standard Deviation
AMPK
6
 0
BRSK2 16 0
BTK
2
 0
CAMK1
7
 0
CAMKK beta
6
 1
CDK2-Cyclin A
5
 0
CHK1
9
 1
CHK2
4
 0
CK1 delta 34 2
CK2 59 4
CSK 19 6
DYRK1A 14 1
DYRK2 31 4
DYRK3 48 1
EF2K 82 1
EPH-A2 11 1
EPH-B3 25 8
ERK1 41 2
ERK2 83 4
ERK8
2
 0
FGF-R1
2
 0
GSK3 beta
8
 6
HIPK2
6
 3
IGF-1R
6
 2
IKK beta 68 6
IKK epsilon 11 8
IR 20 3
IRR 17 3
JNK1 48 3
JNK2 46 2
Lck
5
 2
MAPKAP-K2 33 27
MARK3
10
 1
MELK
4
 1
MKK1 34 5
MNK1 56 1
MNK2 58 6
MSK1
7
 1
MST2
8
 5
MST4 11 0
NEK2a 81 5
NEK6 13 2
p38 alpha MAPK 54 10
p38 beta MAPK 31 2
p38 delta MAPK 53 11
p38 gamma MAPK 43 1
PAK4
7
 2
PAK5
9
 1
PAK6 22 0
PDK1
4
 0
PHK
1
 0
PIM1 22 1
PIM2 14 4
PIM3
5
 2
PKA 28 2
PKB alpha 67 4
PKB beta 24 12
PKC alpha 25 0
PKC zeta 47 5
PKD1 52 8
PLK1 36 0
PRAK 23 14
PRK2 46 5
ROCK 2 14 0
RSK1
9
 5
RSK2 36 0
S6K1
3
 0
SGK1
0
 0
SmMLCK 14 2
Src
5
 0
SRPK1 57 0
SYK
2
 0
TBK1
6
 0
VEGFR1
2
 1
YES1
4
 0