Inhibitor | BI 2536  

Screen Conc: 10.00µM

Kinase	% Activity Remaining Sort descending	Standard Deviation
NEK2a	4	2
PLK1	7	1
MELK	9	2
PKD1	10	1
CAMKK beta	14	1
SmMLCK	14	3
IGF-1R	19	2
CHK2	26	3
RSK2	27	5
CK1 delta	27	0
PHK	29	3
RSK1	30	4
HIPK2	36	1
Lck	39	4
NEK6	43	3
IRAK4	44	3
CAMK1	47	1
DYRK1A	54	0
SYK	54	4
MINK1	55	3
TTK	66	21
SRPK1	66	1
IR	66	1
GCK	67	4
YES1	68	7
IRR	70	0
MKK1	72	6
DYRK2	73	11
EF2K	73	3
MAPKAP-K2	74	4
PIM3	74	1
ERK1	74	2
CDK2-Cyclin A	75	7
EPH-A2	76	7
IKK beta	76	3
CHK1	77	1
S6K1	77	10
HER4	77	1
CK2	78	3
PAK6	79	5
Src	80	1
MLK1	80	16
PKB alpha	81	1
FGF-R1	81	5
PKA	81	0
EPH-B3	82	5
PKC alpha	83	2
LKB1	83	7
SGK1	84	3
JNK1	84	5
JNK2	84	1
p38 beta MAPK	84	4
PKB beta	85	2
MSK1	85	0
AMPK	86	3
Aurora B	89	1
ROCK 2	89	3
p38 alpha MAPK	90	1
TBK1	90	1
MNK1	91	9
PDK1	91	6
ERK2	92	11
PIM1	92	4
PRAK	93	4
BTK	93	8
IKK epsilon	94	5
PRK2	95	4
CSK	95	7
DYRK3	95	2
PKC zeta	95	12
BRSK2	95	6
PAK5	95	7
p38 delta MAPK	96	4
MLK3	97	5
MNK2	98	11
MST4	100	5
GSK3 beta	101	14
p38 gamma MAPK	101	13
MST2	102	5
MARK3	106	4
PAK4	113	3
VEGFR1	114	1
ERK8	115	6
NUAK1	116	10
PIM2	128	4

Screen Conc: 1.00µM

Kinase	% Activity Remaining Sort descending	Standard Deviation
PLK1	6	1
CAMKK beta	16	4
CLK2	21	1
SmMLCK	23	0
EIF2AK3	24	0
MELK	48	1
PKD1	48	2
CHK2	58	4
DAPK1	60	2
IR	61	4
NEK2a	62	1
SYK	63	7
RSK2	69	4
RSK1	73	6
Aurora B	73	1
OSR1	73	2
NUAK1	75	1
SRPK1	77	11
MINK1	77	1
STK33	78	18
TSSK1	78	3
IRAK4	79	2
Src	79	5
TAK1	80	3
PHK	80	3
SIK3	82	5
TBK1	82	2
MKK6	83	1
MARK4	83	9
CDK9-Cyclin T1	84	1
IRR	85	7
LKB1	85	2
NEK6	85	10
PRK2	85	7
MLK3	86	5
IGF-1R	86	7
Lck	88	6
MST4	88	8
CK1 delta	88	10
EPH-A2	88	7
CK1 gamma 2	89	3
DYRK1A	89	8
CAMK1	89	3
ROCK 2	89	4
MAPKAP-K2	90	2
PKB beta	90	6
PAK5	90	0
MEKK1	90	5
JNK3	90	0
GSK3 beta	90	4
EPH-A4	91	3
MST2	91	1
PAK2	92	8
TrkA	92	16
MNK2	92	7
GCK	92	2
DYRK2	92	6
MARK3	92	1
JAK2	92	3
IKK beta	92	4
BRSK2	92	2
TIE2	93	11
JNK1	93	1
MARK2	93	5
JNK2	93	10
MPSK1	93	10
MST3	93	15
ERK2	94	3
DYRK3	94	4
PAK6	94	13
HIPK2	94	5
p38 alpha MAPK	94	12
MLK1	94	2
ZAP70	94	0
PAK4	95	2
IRAK1	96	5
TTK	96	3
PRAK	97	15
AMPK	97	7
ABL	97	9
TAO1	97	10
PIM3	98	2
MAPKAP-K3	98	8
MARK1	98	2
CHK1	98	9
CK2	99	4
TESK1	99	7
RIPK2	99	8
VEGFR1	99	3
IKK epsilon	99	2
MKK1	99	13
p38 delta MAPK	100	15
EF2K	100	2
EPH-B1	100	1
HIPK3	100	7
WNK1	100	4
CSK	101	2
PKA	101	3
BTK	102	6
BRSK1	102	10
PIM1	102	4
EPH-B4	102	4
ERK1	103	3
CDK2-Cyclin A	103	2
MKK2	104	0
DDR2	104	2
PDK1	104	10
p38 gamma MAPK	104	3
MSK1	105	1
TTBK1	105	4
HER4	105	8
PIM2	105	11
PKB alpha	106	4
S6K1	106	4
PKC gamma	106	4
p38 beta MAPK	106	12
SGK1	108	5
ERK8	109	15
YES1	109	7
HIPK1	109	0
PKC zeta	109	8
SIK2	111	7
MNK1	112	2
EPH-B3	114	2
FGF-R1	115	2
TLK1	115	1
PKC alpha	117	10
BRK	118	5
Aurora A	119	11
ASK1	120	12
EPH-B2	140	21

Screen Conc: 0.10µM

Kinase	% Activity Remaining Sort descending	Standard Deviation
PLK1	6	1
CAMKK beta	24	6
EIF2AK3	64	3
CLK2	64	10
SmMLCK	69	0
IGF-1R	73	6
CK1 gamma 2	76	13
CK1 delta	76	3
MELK	78	6
IR	79	6
Aurora B	80	11
SYK	80	11
MST2	81	0
MARK3	82	1
ROCK 2	83	3
TAK1	83	0
MST4	83	10
SIK2	83	9
GSK3 beta	84	1
BTK	85	8
JNK1	85	6
PIM1	85	10
MINK1	85	2
PAK2	85	4
PAK4	86	1
TSSK1	87	5
DAPK1	88	6
CK2	88	1
EPH-B1	88	1
IRAK4	88	5
SIK3	88	4
MLK3	89	6
PHK	89	2
EF2K	89	8
NEK2a	90	10
CHK2	90	4
PKD1	90	12
CDK2-Cyclin A	90	2
MARK1	90	6
TESK1	91	6
IKK epsilon	91	4
CSK	91	11
SRPK1	92	10
TAO1	92	1
ZAP70	92	1
IKK beta	92	9
MAPKAP-K2	92	7
ERK1	92	6
TTK	93	1
MARK4	93	3
PAK5	93	5
MNK2	93	9
ASK1	93	8
BRSK2	93	5
Src	93	5
HIPK2	93	3
MKK2	93	10
MARK2	93	3
ERK2	94	8
JNK2	94	1
PRK2	94	2
LKB1	94	10
S6K1	94	14
DYRK3	94	9
MEKK1	94	19
RSK1	95	1
MKK1	95	8
TTBK1	95	5
AMPK	95	6
CDK9-Cyclin T1	95	6
GCK	95	9
MSK1	96	13
MPSK1	96	9
JNK3	96	2
EPH-A2	96	9
PRAK	96	4
PKB beta	97	5
NUAK1	97	5
HIPK3	97	9
DYRK2	97	4
PIM2	97	1
STK33	97	9
PKA	98	7
PAK6	98	12
WNK1	98	7
TIE2	98	4
RIPK2	98	12
MLK1	99	6
MAPKAP-K3	99	13
IRAK1	99	3
MKK6	99	11
p38 gamma MAPK	99	6
p38 delta MAPK	99	1
CAMK1	99	6
Aurora A	100	2
TLK1	100	1
DDR2	100	2
SGK1	101	4
p38 alpha MAPK	102	6
DYRK1A	102	4
PDK1	103	11
PKB alpha	103	4
VEGFR1	103	1
PIM3	103	12
RSK2	104	3
HIPK1	104	4
PKC zeta	104	0
OSR1	104	7
ABL	104	4
IRR	105	5
CHK1	105	12
JAK2	106	11
TBK1	106	1
EPH-B4	106	9
ERK8	107	2
NEK6	107	1
PKC gamma	107	8
HER4	107	8
Lck	108	8
FGF-R1	108	6
BRSK1	109	1
MST3	109	19
p38 beta MAPK	109	3
YES1	109	0
TrkA	110	14
EPH-B2	110	7
MNK1	112	2
PKC alpha	113	5
EPH-A4	115	1
EPH-B3	115	1
Aurora C	116	3
BRK	124	1