Inhibitor | BI 2536  

Screen Conc: 0.10µM

Kinase	% Activity Remaining	Standard Deviation
ABL	104	4
AMPK	95	6
ASK1	93	8
Aurora A	100	2
Aurora B	80	11
Aurora C	116	3
BRK	124	1
BRSK1	109	1
BRSK2	93	5
BTK	85	8
CAMK1	99	6
CAMKK beta	24	6
CDK2-Cyclin A	90	2
CDK9-Cyclin T1	95	6
CHK1	105	12
CHK2	90	4
CK1 delta	76	3
CK1 gamma 2	76	13
CK2	88	1
CLK2	64	10
CSK	91	11
DAPK1	88	6
DDR2	100	2
DYRK1A	102	4
DYRK2	97	4
DYRK3	94	9
EF2K	89	8
EIF2AK3	64	3
EPH-A2	96	9
EPH-A4	115	1
EPH-B1	88	1
EPH-B2	110	7
EPH-B3	115	1
EPH-B4	106	9
ERK1	92	6
ERK2	94	8
ERK8	107	2
FGF-R1	108	6
GCK	95	9
GSK3 beta	84	1
HER4	107	8
HIPK1	104	4
HIPK2	93	3
HIPK3	97	9
IGF-1R	73	6
IKK beta	92	9
IKK epsilon	91	4
IR	79	6
IRAK1	99	3
IRAK4	88	5
IRR	105	5
JAK2	106	11
JNK1	85	6
JNK2	94	1
JNK3	96	2
Lck	108	8
LKB1	94	10
MAPKAP-K2	92	7
MAPKAP-K3	99	13
MARK1	90	6
MARK2	93	3
MARK3	82	1
MARK4	93	3
MEKK1	94	19
MELK	78	6
MINK1	85	2
MKK1	95	8
MKK2	93	10
MKK6	99	11
MLK1	99	6
MLK3	89	6
MNK1	112	2
MNK2	93	9
MPSK1	96	9
MSK1	96	13
MST2	81	0
MST3	109	19
MST4	83	10
NEK2a	90	10
NEK6	107	1
NUAK1	97	5
OSR1	104	7
p38 alpha MAPK	102	6
p38 beta MAPK	109	3
p38 delta MAPK	99	1
p38 gamma MAPK	99	6
PAK2	85	4
PAK4	86	1
PAK5	93	5
PAK6	98	12
PDK1	103	11
PHK	89	2
PIM1	85	10
PIM2	97	1
PIM3	103	12
PKA	98	7
PKB alpha	103	4
PKB beta	97	5
PKC alpha	113	5
PKC gamma	107	8
PKC zeta	104	0
PKD1	90	12
PLK1	6	1
PRAK	96	4
PRK2	94	2
RIPK2	98	12
ROCK 2	83	3
RSK1	95	1
RSK2	104	3
S6K1	94	14
SGK1	101	4
SIK2	83	9
SIK3	88	4
SmMLCK	69	0
Src	93	5
SRPK1	92	10
STK33	97	9
SYK	80	11
TAK1	83	0
TAO1	92	1
TBK1	106	1
TESK1	91	6
TIE2	98	4
TLK1	100	1
TrkA	110	14
TSSK1	87	5
TTBK1	95	5
TTK	93	1
VEGFR1	103	1
WNK1	98	7
YES1	109	0
ZAP70	92	1

Screen Conc: 1.00µM

Kinase	% Activity Remaining	Standard Deviation
ABL	97	9
AMPK	97	7
ASK1	120	12
Aurora A	119	11
Aurora B	73	1
BRK	118	5
BRSK1	102	10
BRSK2	92	2
BTK	102	6
CAMK1	89	3
CAMKK beta	16	4
CDK2-Cyclin A	103	2
CDK9-Cyclin T1	84	1
CHK1	98	9
CHK2	58	4
CK1 delta	88	10
CK1 gamma 2	89	3
CK2	99	4
CLK2	21	1
CSK	101	2
DAPK1	60	2
DDR2	104	2
DYRK1A	89	8
DYRK2	92	6
DYRK3	94	4
EF2K	100	2
EIF2AK3	24	0
EPH-A2	88	7
EPH-A4	91	3
EPH-B1	100	1
EPH-B2	140	21
EPH-B3	114	2
EPH-B4	102	4
ERK1	103	3
ERK2	94	3
ERK8	109	15
FGF-R1	115	2
GCK	92	2
GSK3 beta	90	4
HER4	105	8
HIPK1	109	0
HIPK2	94	5
HIPK3	100	7
IGF-1R	86	7
IKK beta	92	4
IKK epsilon	99	2
IR	61	4
IRAK1	96	5
IRAK4	79	2
IRR	85	7
JAK2	92	3
JNK1	93	1
JNK2	93	10
JNK3	90	0
Lck	88	6
LKB1	85	2
MAPKAP-K2	90	2
MAPKAP-K3	98	8
MARK1	98	2
MARK2	93	5
MARK3	92	1
MARK4	83	9
MEKK1	90	5
MELK	48	1
MINK1	77	1
MKK1	99	13
MKK2	104	0
MKK6	83	1
MLK1	94	2
MLK3	86	5
MNK1	112	2
MNK2	92	7
MPSK1	93	10
MSK1	105	1
MST2	91	1
MST3	93	15
MST4	88	8
NEK2a	62	1
NEK6	85	10
NUAK1	75	1
OSR1	73	2
p38 alpha MAPK	94	12
p38 beta MAPK	106	12
p38 delta MAPK	100	15
p38 gamma MAPK	104	3
PAK2	92	8
PAK4	95	2
PAK5	90	0
PAK6	94	13
PDK1	104	10
PHK	80	3
PIM1	102	4
PIM2	105	11
PIM3	98	2
PKA	101	3
PKB alpha	106	4
PKB beta	90	6
PKC alpha	117	10
PKC gamma	106	4
PKC zeta	109	8
PKD1	48	2
PLK1	6	1
PRAK	97	15
PRK2	85	7
RIPK2	99	8
ROCK 2	89	4
RSK1	73	6
RSK2	69	4
S6K1	106	4
SGK1	108	5
SIK2	111	7
SIK3	82	5
SmMLCK	23	0
Src	79	5
SRPK1	77	11
STK33	78	18
SYK	63	7
TAK1	80	3
TAO1	97	10
TBK1	82	2
TESK1	99	7
TIE2	93	11
TLK1	115	1
TrkA	92	16
TSSK1	78	3
TTBK1	105	4
TTK	96	3
VEGFR1	99	3
WNK1	100	4
YES1	109	7
ZAP70	94	0

Screen Conc: 10.00µM

Kinase	% Activity Remaining	Standard Deviation
AMPK	86	3
Aurora B	89	1
BRSK2	95	6
BTK	93	8
CAMK1	47	1
CAMKK beta	14	1
CDK2-Cyclin A	75	7
CHK1	77	1
CHK2	26	3
CK1 delta	27	0
CK2	78	3
CSK	95	7
DYRK1A	54	0
DYRK2	73	11
DYRK3	95	2
EF2K	73	3
EPH-A2	76	7
EPH-B3	82	5
ERK1	74	2
ERK2	92	11
ERK8	115	6
FGF-R1	81	5
GCK	67	4
GSK3 beta	101	14
HER4	77	1
HIPK2	36	1
IGF-1R	19	2
IKK beta	76	3
IKK epsilon	94	5
IR	66	1
IRAK4	44	3
IRR	70	0
JNK1	84	5
JNK2	84	1
Lck	39	4
LKB1	83	7
MAPKAP-K2	74	4
MARK3	106	4
MELK	9	2
MINK1	55	3
MKK1	72	6
MLK1	80	16
MLK3	97	5
MNK1	91	9
MNK2	98	11
MSK1	85	0
MST2	102	5
MST4	100	5
NEK2a	4	2
NEK6	43	3
NUAK1	116	10
p38 alpha MAPK	90	1
p38 beta MAPK	84	4
p38 delta MAPK	96	4
p38 gamma MAPK	101	13
PAK4	113	3
PAK5	95	7
PAK6	79	5
PDK1	91	6
PHK	29	3
PIM1	92	4
PIM2	128	4
PIM3	74	1
PKA	81	0
PKB alpha	81	1
PKB beta	85	2
PKC alpha	83	2
PKC zeta	95	12
PKD1	10	1
PLK1	7	1
PRAK	93	4
PRK2	95	4
ROCK 2	89	3
RSK1	30	4
RSK2	27	5
S6K1	77	10
SGK1	84	3
SmMLCK	14	3
Src	80	1
SRPK1	66	1
SYK	54	4
TBK1	90	1
TTK	66	21
VEGFR1	114	1
YES1	68	7