Inhibitor | KU 55933  

Screen Conc: 0.01µM

Kinase	% Activity Remaining	Standard Deviation
AMPK	111	9
Aurora A	116	3
Aurora B	94	7
BRSK1	99	10
BRSK2	117	3
BTK	91	9
CAMK1	103	3
CAMKK beta	128	16
CDK2-Cyclin A	105	15
CHK1	92	1
CHK2	120	10
CK1 delta	133	7
CK2	103	1
CSK	142	12
DYRK1A	98	0
DYRK2	93	2
DYRK3	61	2
EF2K	99	12
EPH-A2	127	9
EPH-A4	114	1
EPH-B3	100	1
EPH-B4	125	5
ERK1	123	12
ERK2	109	8
ERK8	106	2
FGF-R1	121	2
GCK	72	3
GSK3 beta	108	12
HER4	118	1
HIPK1	118	9
HIPK2	108	5
HIPK3	119	20
IGF-1R	101	2
IKK beta	90	9
IKK epsilon	81	2
IR	107	3
IRAK4	66	7
IRR	102	11
JAK2	117	6
JNK1	112	13
JNK2	113	5
JNK3	119	15
Lck	109	4
LKB1	95	10
MAPKAP-K2	131	26
MAPKAP-K3	123	2
MARK1	87	14
MARK2	116	9
MARK3	82	7
MARK4	111	7
MEKK1	99	0
MELK	81	4
MINK1	77	3
MKK1	90	9
MLK1	80	8
MLK3	56	6
MNK1	144	42
MNK2	146	29
MSK1	97	18
MST2	93	6
MST4	137	9
NEK2a	101	6
NEK6	114	1
NUAK1	89	11
p38 alpha MAPK	98	4
p38 beta MAPK	108	7
p38 delta MAPK	100	22
p38 gamma MAPK	111	0
PAK2	124	4
PAK4	110	2
PAK5	129	2
PAK6	104	7
PDK1	134	3
PHK	78	4
PIM1	106	4
PIM2	91	21
PIM3	101	2
PKA	101	0
PKB alpha	100	2
PKB beta	107	14
PKC alpha	119	10
PKC zeta	112	3
PKD1	105	10
PLK1	120	3
PRAK	126	18
PRK2	83	10
RIPK2	105	4
ROCK 2	111	5
RSK1	98	7
RSK2	112	5
S6K1	112	6
SGK1	116	9
SmMLCK	116	7
Src	98	1
SRPK1	107	6
SYK	119	28
TAK1	109	8
TBK1	109	12
TrkA	104	39
TTK	117	8
VEGFR1	122	25
YES1	108	7

Screen Conc: 0.10µM

Kinase	% Activity Remaining	Standard Deviation
AMPK	97	7
Aurora A	121	1
Aurora B	97	2
BRSK1	100	8
BRSK2	110	10
BTK	86	5
CAMK1	92	1
CAMKK beta	100	30
CDK2-Cyclin A	102	3
CHK1	66	5
CHK2	87	5
CK1 delta	93	5
CK2	94	2
CSK	101	10
DYRK1A	77	1
DYRK2	71	7
DYRK3	83	33
EF2K	81	21
EPH-A2	82	18
EPH-A4	109	3
EPH-B3	86	4
EPH-B4	83	3
ERK1	105	4
ERK2	84	0
ERK8	101	2
FGF-R1	105	5
GCK	62	1
GSK3 beta	78	5
HER4	99	1
HIPK1	93	1
HIPK2	90	2
HIPK3	92	10
IGF-1R	94	10
IKK beta	65	2
IKK epsilon	50	3
IR	107	6
IRAK4	58	0
IRR	78	10
JAK2	85	10
JNK1	96	4
JNK2	86	1
JNK3	91	2
Lck	65	34
LKB1	94	6
MAPKAP-K2	112	5
MAPKAP-K3	86	3
MARK1	74	6
MARK2	91	0
MARK3	65	14
MARK4	77	3
MEKK1	67	19
MELK	73	3
MINK1	69	7
MKK1	62	9
MLK1	66	10
MLK3	38	5
MNK1	113	8
MNK2	76	13
MSK1	70	8
MST2	66	8
MST4	111	16
NEK2a	74	10
NEK6	78	11
NUAK1	89	1
p38 alpha MAPK	67	10
p38 beta MAPK	90	1
p38 delta MAPK	83	20
p38 gamma MAPK	91	2
PAK2	89	1
PAK4	90	13
PAK5	96	3
PAK6	82	12
PDK1	114	5
PHK	61	0
PIM1	88	4
PIM2	82	2
PIM3	51	6
PKA	90	10
PKB alpha	65	5
PKB beta	90	5
PKC alpha	102	10
PKC zeta	99	11
PKD1	88	12
PLK1	95	21
PRAK	90	7
PRK2	73	1
RIPK2	96	1
ROCK 2	81	10
RSK1	78	2
RSK2	94	4
S6K1	91	4
SGK1	80	11
SmMLCK	93	10
Src	93	2
SRPK1	78	0
SYK	108	23
TAK1	80	0
TBK1	90	8
TrkA	78	12
TTK	106	5
VEGFR1	92	15
YES1	103	11

Screen Conc: 1.00µM

Kinase	% Activity Remaining	Standard Deviation
AMPK	96	5
ASK1	92	13
Aurora A	109	3
Aurora B	115	4
BRSK1	104	3
BRSK2	113	3
BTK	97	3
CAMK1	103	5
CAMKK beta	94	3
CDK2-Cyclin A	98	8
CHK1	98	14
CHK2	123	10
CK1 delta	112	4
CK2	101	10
CLK2	88	10
CSK	93	1
DAPK1	81	8
DYRK1A	66	3
DYRK2	93	1
DYRK3	84	3
EF2K	66	5
EPH-A2	104	1
EPH-A4	101	8
EPH-B1	100	6
EPH-B2	107	1
EPH-B3	100	6
EPH-B4	106	2
ERK1	96	4
ERK2	100	8
ERK8	84	6
FGF-R1	97	12
GCK	100	5
GSK3 beta	66	2
HER4	83	7
HIPK1	97	6
HIPK2	83	4
HIPK3	109	7
IGF-1R	74	3
IKK beta	94	14
IKK epsilon	88	1
IR	100	4
IRAK4	73	7
IRR	87	3
JAK2	89	3
JNK1	106	0
JNK2	103	5
JNK3	87	10
Lck	109	2
LKB1	95	4
MAPKAP-K2	97	4
MAPKAP-K3	99	2
MARK1	99	6
MARK2	101	14
MARK3	107	3
MARK4	99	11
MEKK1	109	9
MELK	87	6
MINK1	94	7
MKK1	81	7
MKK2	94	5
MKK6	104	3
MLK1	97	4
MLK3	99	5
MNK1	104	7
MNK2	88	7
MSK1	94	7
MST2	82	2
MST4	102	2
NEK2a	105	7
NEK6	104	1
NUAK1	88	1
p38 alpha MAPK	113	0
p38 beta MAPK	96	8
p38 delta MAPK	87	7
p38 gamma MAPK	102	6
PAK2	91	12
PAK4	96	9
PAK5	100	1
PAK6	108	5
PDK1	88	3
PHK	91	9
PIM1	56	12
PIM2	84	7
PIM3	31	1
PKA	105	1
PKB alpha	92	3
PKB beta	90	14
PKC alpha	104	2
PKC zeta	104	8
PKD1	89	1
PLK1	89	7
PRAK	85	2
PRK2	101	4
RIPK2	99	1
ROCK 2	96	4
RSK1	101	5
RSK2	101	7
S6K1	98	1
SGK1	103	7
SmMLCK	96	3
Src	99	5
SRPK1	106	12
SYK	97	0
TAK1	113	1
TAO1	94	5
TBK1	105	12
TrkA	95	3
TTK	74	2
VEGFR1	96	8
YES1	96	8

Screen Conc: 10.00µM

Kinase	% Activity Remaining	Standard Deviation
AMPK	88	8
ASK1	76	5
Aurora A	94	8
Aurora B	81	3
BRSK1	115	1
BRSK2	91	13
BTK	62	1
CAMK1	64	6
CAMKK beta	72	12
CDK2-Cyclin A	61	8
CHK1	104	11
CHK2	97	9
CK1 delta	97	11
CK2	92	3
CLK2	65	10
CSK	90	3
DAPK1	84	3
DYRK1A	19	1
DYRK2	86	9
DYRK3	78	4
EF2K	43	3
EPH-A2	98	3
EPH-A4	95	11
EPH-B1	110	0
EPH-B2	108	3
EPH-B3	103	4
EPH-B4	129	5
ERK1	109	7
ERK2	101	0
ERK8	28	2
FGF-R1	94	10
GCK	80	4
GSK3 beta	26	0
HER4	91	1
HIPK1	82	3
HIPK2	52	8
HIPK3	78	0
IGF-1R	63	11
IKK beta	90	5
IKK epsilon	84	1
IR	102	13
IRAK4	79	10
IRR	60	5
JAK2	95	1
JNK1	98	1
JNK2	94	0
JNK3	115	2
Lck	94	11
LKB1	88	1
MAPKAP-K2	97	13
MAPKAP-K3	93	10
MARK1	104	12
MARK2	94	3
MARK3	82	2
MARK4	100	4
MEKK1	94	12
MELK	82	7
MINK1	81	11
MKK1	59	2
MKK2	95	2
MKK6	118	13
MLK1	88	3
MLK3	90	7
MNK1	115	2
MNK2	89	3
MSK1	78	5
MST2	83	3
MST4	63	3
NEK2a	85	6
NEK6	107	14
NUAK1	76	2
p38 alpha MAPK	95	1
p38 beta MAPK	120	3
p38 delta MAPK	104	7
p38 gamma MAPK	85	14
PAK2	90	1
PAK4	97	1
PAK5	67	11
PAK6	79	2
PDK1	92	4
PHK	88	7
PIM1	13	0
PIM2	35	4
PIM3	19	0
PKA	89	1
PKB alpha	100	1
PKB beta	61	8
PKC alpha	73	2
PKC zeta	87	11
PKD1	58	8
PLK1	78	1
PRAK	43	0
PRK2	94	9
RIPK2	97	2
ROCK 2	83	2
RSK1	78	5
RSK2	84	13
S6K1	86	1
SGK1	106	1
SmMLCK	51	2
Src	110	15
SRPK1	98	7
SYK	94	11
TAK1	63	4
TAO1	90	3
TBK1	78	2
TrkA	97	2
TTK	74	0
VEGFR1	80	6
YES1	112	9