Inhibitor |
HA-1100 (Hydroxyfasudil)
Screening Results Tables
Screen Conc:
1.00µM
| Kinase |
% Activity Remaining |
Standard Deviation |
|
AMPK
|
92
|
4
|
|
ASK1
|
101
|
5
|
|
Aurora A
|
99
|
6
|
|
Aurora B
|
110
|
8
|
|
BRSK1
|
95
|
10
|
|
BRSK2
|
106
|
5
|
|
BTK
|
90
|
7
|
|
CAMK1
|
95
|
6
|
|
CAMKK beta
|
92
|
11
|
|
CDK2-Cyclin A
|
104
|
2
|
|
CHK1
|
103
|
8
|
|
CHK2
|
93
|
6
|
|
CK1 delta
|
97
|
10
|
|
CK2
|
104
|
2
|
|
CLK2
|
85
|
1
|
|
CSK
|
90
|
4
|
|
DAPK1
|
79
|
1
|
|
DYRK1A
|
101
|
5
|
|
DYRK2
|
80
|
2
|
|
DYRK3
|
103
|
8
|
|
EF2K
|
95
|
11
|
|
EPH-A2
|
92
|
7
|
|
EPH-A4
|
94
|
5
|
|
EPH-B1
|
101
|
6
|
|
EPH-B2
|
104
|
13
|
|
EPH-B3
|
101
|
9
|
|
EPH-B4
|
101
|
5
|
|
ERK1
|
90
|
3
|
|
ERK2
|
90
|
1
|
|
ERK8
|
97
|
2
|
|
FGF-R1
|
93
|
6
|
|
GCK
|
91
|
1
|
|
GSK3 beta
|
86
|
6
|
|
HER4
|
96
|
14
|
|
HIPK1
|
87
|
2
|
|
HIPK2
|
105
|
6
|
|
HIPK3
|
90
|
10
|
|
IGF-1R
|
109
|
11
|
|
IKK beta
|
87
|
7
|
|
IKK epsilon
|
88
|
3
|
|
IR
|
106
|
1
|
|
IRAK4
|
84
|
7
|
|
IRR
|
94
|
4
|
|
JAK2
|
91
|
5
|
|
JNK1
|
103
|
8
|
|
JNK2
|
99
|
3
|
|
JNK3
|
99
|
5
|
|
Lck
|
95
|
2
|
|
LKB1
|
81
|
9
|
|
MAPKAP-K2
|
101
|
15
|
|
MAPKAP-K3
|
92
|
10
|
|
MARK1
|
87
|
6
|
|
MARK2
|
80
|
5
|
|
MARK3
|
92
|
7
|
|
MARK4
|
97
|
6
|
|
MEKK1
|
94
|
6
|
|
MELK
|
92
|
5
|
|
MINK1
|
94
|
4
|
|
MKK1
|
91
|
11
|
|
MKK2
|
83
|
1
|
|
MKK6
|
84
|
7
|
|
MLK1
|
90
|
8
|
|
MLK3
|
87
|
3
|
|
MNK1
|
103
|
2
|
|
MNK2
|
104
|
1
|
|
MSK1
|
59
|
5
|
|
MST2
|
80
|
12
|
|
MST4
|
98
|
9
|
|
NEK2a
|
83
|
1
|
|
NEK6
|
110
|
8
|
|
NUAK1
|
67
|
5
|
|
p38 alpha MAPK
|
69
|
7
|
|
p38 beta MAPK
|
96
|
9
|
|
p38 delta MAPK
|
83
|
7
|
|
p38 gamma MAPK
|
95
|
11
|
|
PAK2
|
94
|
2
|
|
PAK4
|
91
|
4
|
|
PAK5
|
88
|
3
|
|
PAK6
|
85
|
3
|
|
PDK1
|
98
|
0
|
|
PHK
|
113
|
1
|
|
PIM1
|
95
|
8
|
|
PIM2
|
96
|
4
|
|
PIM3
|
99
|
5
|
|
PKA
|
95
|
6
|
|
PKB alpha
|
85
|
2
|
|
PKB beta
|
91
|
9
|
|
PKC alpha
|
82
|
5
|
|
PKC zeta
|
92
|
4
|
|
PKD1
|
97
|
7
|
|
PLK1
|
96
|
6
|
|
PRAK
|
93
|
3
|
|
PRK2
|
67
|
0
|
|
RIPK2
|
97
|
6
|
|
ROCK 2
|
43
|
3
|
|
RSK1
|
52
|
2
|
|
RSK2
|
78
|
2
|
|
S6K1
|
55
|
1
|
|
SGK1
|
104
|
10
|
|
SmMLCK
|
83
|
5
|
|
Src
|
102
|
15
|
|
SRPK1
|
93
|
1
|
|
SYK
|
88
|
2
|
|
TAK1
|
87
|
0
|
|
TAO1
|
84
|
9
|
|
TBK1
|
90
|
1
|
|
TrkA
|
100
|
1
|
|
TTK
|
100
|
6
|
|
VEGFR1
|
82
|
11
|
|
YES1
|
102
|
2
|
Screen Conc:
10.00µM
| Kinase |
% Activity Remaining |
Standard Deviation |
|
AMPK
|
89
|
9
|
|
ASK1
|
95
|
7
|
|
Aurora A
|
89
|
7
|
|
Aurora B
|
62
|
11
|
|
BRSK1
|
63
|
10
|
|
BRSK2
|
74
|
1
|
|
BTK
|
102
|
5
|
|
CAMK1
|
86
|
5
|
|
CAMKK beta
|
74
|
0
|
|
CDK2-Cyclin A
|
119
|
0
|
|
CHK1
|
86
|
0
|
|
CHK2
|
96
|
7
|
|
CK1 delta
|
101
|
6
|
|
CK2
|
89
|
12
|
|
CLK2
|
49
|
0
|
|
CSK
|
88
|
2
|
|
DAPK1
|
107
|
11
|
|
DYRK1A
|
110
|
7
|
|
DYRK2
|
102
|
2
|
|
DYRK3
|
101
|
4
|
|
EF2K
|
96
|
2
|
|
EPH-A2
|
81
|
13
|
|
EPH-A4
|
105
|
6
|
|
EPH-B1
|
99
|
6
|
|
EPH-B2
|
104
|
6
|
|
EPH-B3
|
84
|
6
|
|
EPH-B4
|
80
|
4
|
|
ERK1
|
106
|
15
|
|
ERK2
|
102
|
7
|
|
ERK8
|
81
|
1
|
|
FGF-R1
|
83
|
4
|
|
GCK
|
92
|
3
|
|
GSK3 beta
|
94
|
8
|
|
HER4
|
74
|
3
|
|
HIPK1
|
104
|
7
|
|
HIPK2
|
96
|
3
|
|
HIPK3
|
92
|
3
|
|
IGF-1R
|
85
|
1
|
|
IKK beta
|
104
|
4
|
|
IKK epsilon
|
93
|
4
|
|
IR
|
102
|
7
|
|
IRAK4
|
87
|
1
|
|
IRR
|
108
|
12
|
|
JAK2
|
87
|
4
|
|
JNK1
|
96
|
2
|
|
JNK2
|
100
|
5
|
|
JNK3
|
103
|
3
|
|
Lck
|
99
|
1
|
|
LKB1
|
75
|
5
|
|
MAPKAP-K2
|
94
|
3
|
|
MAPKAP-K3
|
91
|
1
|
|
MARK1
|
77
|
4
|
|
MARK2
|
71
|
3
|
|
MARK3
|
90
|
6
|
|
MARK4
|
92
|
6
|
|
MEKK1
|
57
|
1
|
|
MELK
|
88
|
5
|
|
MINK1
|
87
|
1
|
|
MKK1
|
84
|
12
|
|
MKK2
|
83
|
2
|
|
MKK6
|
88
|
1
|
|
MLK1
|
95
|
7
|
|
MLK3
|
85
|
2
|
|
MNK1
|
92
|
3
|
|
MNK2
|
101
|
7
|
|
MSK1
|
23
|
1
|
|
MST2
|
57
|
5
|
|
MST4
|
83
|
3
|
|
NEK2a
|
97
|
14
|
|
NEK6
|
106
|
5
|
|
NUAK1
|
39
|
5
|
|
p38 alpha MAPK
|
103
|
10
|
|
p38 beta MAPK
|
92
|
13
|
|
p38 delta MAPK
|
104
|
10
|
|
p38 gamma MAPK
|
93
|
2
|
|
PAK2
|
95
|
6
|
|
PAK4
|
96
|
6
|
|
PAK5
|
100
|
9
|
|
PAK6
|
86
|
0
|
|
PDK1
|
96
|
3
|
|
PHK
|
104
|
3
|
|
PIM1
|
78
|
1
|
|
PIM2
|
103
|
1
|
|
PIM3
|
75
|
5
|
|
PKA
|
83
|
4
|
|
PKB alpha
|
60
|
3
|
|
PKB beta
|
76
|
3
|
|
PKC alpha
|
83
|
2
|
|
PKC zeta
|
92
|
3
|
|
PKD1
|
79
|
2
|
|
PLK1
|
89
|
3
|
|
PRAK
|
93
|
1
|
|
PRK2
|
32
|
5
|
|
RIPK2
|
96
|
4
|
|
ROCK 2
|
13
|
0
|
|
RSK1
|
18
|
1
|
|
RSK2
|
50
|
1
|
|
S6K1
|
27
|
2
|
|
SGK1
|
95
|
1
|
|
SmMLCK
|
91
|
10
|
|
Src
|
89
|
6
|
|
SRPK1
|
104
|
7
|
|
SYK
|
96
|
3
|
|
TAK1
|
47
|
1
|
|
TAO1
|
86
|
0
|
|
TBK1
|
90
|
8
|
|
TrkA
|
70
|
1
|
|
TTK
|
88
|
2
|
|
VEGFR1
|
98
|
12
|
|
YES1
|
82
|
10
|
