Inhibitor | VX-745  

Screen Conc: 0.10µM

Kinase	% Activity Remaining	Standard Deviation
AMPK	100	3
Aurora B	99	18
BRSK2	94	11
BTK	98	7
CAMK1	93	17
CAMKK beta	110	4
CDK2-Cyclin A	101	17
CHK1	113	4
CHK2	106	3
CK1 delta	100	1
CK2	94	4
CSK	115	3
DYRK1A	101	11
DYRK2	108	0
DYRK3	104	0
EF2K	99	31
EPH-A2	123	5
EPH-B3	89	6
ERK1	103	0
ERK2	105	0
ERK8	111	26
FGF-R1	106	4
GCK	104	2
GSK3 beta	112	4
HER4	119	5
HIPK2	107	15
IGF-1R	115	4
IKK beta	98	8
IKK epsilon	105	3
IR	86	9
IRAK4	100	7
IRR	101	4
JNK1	114	19
JNK2	111	6
Lck	103	13
LKB1	96	3
MAPKAP-K2	107	2
MARK3	112	2
MELK	101	13
MINK1	120	2
MKK1	98	2
MLK1	91	3
MLK3	102	10
MNK1	75	1
MNK2	94	1
MSK1	91	6
MST2	132	16
MST4	103	1
NEK2a	111	1
NEK6	101	0
NUAK1	101	3
p38 alpha MAPK	17	6
p38 beta MAPK	66	8
p38 delta MAPK	119	24
p38 gamma MAPK	95	19
PAK4	107	4
PAK5	99	4
PAK6	96	9
PDK1	111	6
PHK	50	3
PIM1	101	16
PIM2	114	9
PIM3	100	13
PKA	103	9
PKB alpha	95	6
PKB beta	93	1
PKC alpha	102	9
PKC zeta	111	0
PKD1	112	19
PLK1	118	15
PRAK	96	8
PRK2	89	14
ROCK 2	96	4
RSK1	109	6
RSK2	85	4
S6K1	112	19
SGK1	127	21
SmMLCK	120	1
Src	93	9
SRPK1	106	9
SYK	114	0
TBK1	108	2
TTK	106	16
VEGFR1	117	2
YES1	103	3

Screen Conc: 1.00µM

Kinase	% Activity Remaining	Standard Deviation
AMPK	97	2
ASK1	105	12
Aurora A	98	7
Aurora B	88	0
BRSK1	103	3
BRSK2	87	8
BTK	102	1
CAMK1	104	8
CAMKK beta	89	5
CDK2-Cyclin A	110	2
CHK1	97	2
CHK2	107	6
CK1 delta	93	5
CK2	106	8
CLK2	81	8
CSK	99	5
DAPK1	86	5
DYRK1A	109	0
DYRK2	103	7
DYRK3	104	1
EF2K	90	1
EPH-A2	89	7
EPH-A4	106	11
EPH-B1	97	1
EPH-B2	94	13
EPH-B3	95	1
EPH-B4	92	2
ERK1	93	7
ERK2	100	15
ERK8	101	6
FGF-R1	105	9
GCK	96	4
GSK3 beta	91	4
HER4	99	6
HIPK1	98	1
HIPK2	91	0
HIPK3	102	2
IGF-1R	89	15
IKK beta	93	2
IKK epsilon	97	1
IR	108	8
IRAK4	81	5
IRR	83	2
JAK2	85	9
JNK1	89	6
JNK2	94	2
JNK3	92	6
Lck	101	11
LKB1	87	6
MAPKAP-K2	96	3
MAPKAP-K3	86	5
MARK1	95	2
MARK2	86	0
MARK3	93	0
MARK4	103	2
MEKK1	95	12
MELK	96	1
MINK1	107	15
MKK1	92	7
MKK2	93	2
MKK6	111	6
MLK1	104	1
MLK3	91	2
MNK1	124	1
MNK2	109	9
MSK1	89	3
MST2	96	2
MST4	92	3
NEK2a	83	3
NEK6	103	15
NUAK1	94	2
p38 alpha MAPK	5	1
p38 beta MAPK	28	5
p38 delta MAPK	77	3
p38 gamma MAPK	84	13
PAK2	98	10
PAK4	93	5
PAK5	94	1
PAK6	95	6
PDK1	93	3
PHK	114	0
PIM1	94	9
PIM2	108	5
PIM3	86	4
PKA	99	11
PKB alpha	87	3
PKB beta	91	7
PKC alpha	97	1
PKC zeta	99	3
PKD1	86	5
PLK1	99	12
PRAK	95	5
PRK2	84	10
RIPK2	102	5
ROCK 2	87	4
RSK1	104	1
RSK2	95	11
S6K1	92	10
SGK1	103	3
SmMLCK	83	3
Src	93	1
SRPK1	97	1
SYK	113	4
TAK1	89	3
TAO1	102	2
TBK1	94	6
TrkA	89	9
TTK	85	1
VEGFR1	100	12
YES1	95	4

Screen Conc: 10.00µM

Kinase	% Activity Remaining	Standard Deviation
AMPK	81	5
ASK1	106	13
Aurora A	103	2
Aurora B	85	9
BRSK1	106	2
BRSK2	88	11
BTK	84	11
CAMK1	86	5
CAMKK beta	86	0
CDK2-Cyclin A	106	3
CHK1	78	2
CHK2	90	6
CK1 delta	99	5
CK2	119	3
CLK2	76	15
CSK	89	7
DAPK1	80	10
DYRK1A	104	10
DYRK2	86	3
DYRK3	95	12
EF2K	84	3
EPH-A2	96	1
EPH-A4	103	4
EPH-B1	92	4
EPH-B2	97	11
EPH-B3	93	9
EPH-B4	95	4
ERK1	95	2
ERK2	84	1
ERK8	109	4
FGF-R1	116	14
GCK	98	2
GSK3 beta	83	3
HER4	99	1
HIPK1	101	2
HIPK2	97	7
HIPK3	102	7
IGF-1R	103	5
IKK beta	95	4
IKK epsilon	95	7
IR	88	15
IRAK4	88	0
IRR	105	7
JAK2	101	7
JNK1	93	2
JNK2	84	7
JNK3	91	1
Lck	89	1
LKB1	93	0
MAPKAP-K2	94	7
MAPKAP-K3	89	2
MARK1	84	4
MARK2	92	4
MARK3	98	2
MARK4	96	3
MEKK1	96	5
MELK	87	8
MINK1	95	3
MKK1	86	7
MKK2	71	1
MKK6	104	4
MLK1	97	6
MLK3	85	10
MNK1	94	2
MNK2	97	7
MSK1	86	2
MST2	95	12
MST4	98	1
NEK2a	85	2
NEK6	97	11
NUAK1	89	4
p38 alpha MAPK	4	0
p38 beta MAPK	20	2
p38 delta MAPK	89	3
p38 gamma MAPK	82	5
PAK2	93	6
PAK4	91	9
PAK5	80	2
PAK6	83	14
PDK1	91	1
PHK	116	3
PIM1	91	8
PIM2	105	4
PIM3	80	3
PKA	90	4
PKB alpha	98	6
PKB beta	91	7
PKC alpha	97	6
PKC zeta	106	6
PKD1	96	7
PLK1	106	5
PRAK	95	5
PRK2	89	6
RIPK2	110	12
ROCK 2	80	6
RSK1	104	3
RSK2	78	1
S6K1	90	6
SGK1	100	12
SmMLCK	74	5
Src	92	15
SRPK1	95	7
SYK	99	2
TAK1	91	10
TAO1	83	3
TBK1	89	7
TrkA	89	11
TTK	97	1
VEGFR1	80	12
YES1	91	6