Longname
c-Jun N-terminal kinase 2
3Letter
JN2
Accession
L31951
Activation
MKK4 and MKK7
Assay Method
JNK2α2 (5-20 mU diluted in 50 mM Tris (pH 7.5), 0.1mM EGTA, 1mg/ml BSA, 0.1% β-mercaptoethanol) is assayed against ATF2 (activating transcription factor in a final volume of 25.5 µl in 50 mM Tris pH 7.5, 0.1 mM EGTA, 0.1% β-mercaptoethanol, ATF2 (3 µM), 10 mM magnesium acetate and 0.02 mM [33P-γ-ATP] (500 -1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
20
Base36
11
Express
Off
ExpressionSys
Sf21 cells
KCode
37
Kinase Type
k
PDF
JNK2_4.pdf
(70.92 KB)
Species
Human
TissRec
R