Longname
Insulin Receptor Related Receptor 1
3Letter
IR1
Accession
NM_014215
Activation
N/A constitutive
Assay Method
5-20mU of IRR (diluted in 50mM Hepes (pH 7.5), 0.1mM EGTA) is assayed against MBP in a final volume of 25.5µl containing 50mM Hepes (pH 7.5), 0.1mM EGTA, 0.33mg/ml MBP, 10 mM magnesium acetate and 0.005 mM [33P-γ-ATP]( 500-1000 cpm/pmole) and incubated for 30 mins at room temperature. Assays are stopped by addition of 5µl of 0.5M (3%) orthophosphoric acid. Assays are harvested onto P81 Unifilter plates using a wash buffer of 50mM orthophosphoric acid.
ATPGroup
5
Base36
29
Express
Off
ExpressionSys
Sf21 cells
KCode
81
Kinase Type
k
PDF
IRR_4.pdf
(75.75 KB)
Species
Human
TissRec
R