Longname
Dual specificity tyrosine phosphorylation Regulated Kinase 3
3Letter
D3A
Accession
AY590695
Activation
N/A constitutive
Assay Method
DYRK3 (5-20 mU of diluted in 50 mM Tris pH7.5, 0.1 mM EGTA) is assayed against Woodtide (KKISGRLSPIMTEQ) in a final volume of 25.5µl containing 50 mM Tris pH 7.5, 0.1 mM EGTA, 350 µM substrate peptide, 10 mM Magnesium acetate and 0.005 mM [33P-γ-ATP](50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
5
Base36
27
Express
Off
ExpressionSys
E.coli
KCode
79
Kinase Type
k
PDF
DYRK3_3.pdf
(82.03 KB)
Species
Human
TissRec
R