Longname
zeta chain (TCR) associated protein kinase 70
3Letter
Z70
Accession
NM_001079
Activation
N/A constitutive
Assay Method
ZAP70 (5-20mU diluted in 50 mM Tris pH 7.5, 0.1 mM EGTA, 1 mg/ml BSA0.1%,β-mercaptoethanol) is assayed against a substrate peptide (Poly Glut Tyr) in a final volume of 25.5 μl containing 50mM Tris pH 7.5, 0.1mMEGTA, 10mM MnCl,1mg/ml substrate peptide, 10 mM magnesium acetate 0.005 mM [33P-γ-ATP] (50-1000cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 μl of0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50mM orthophosphoric acid.
ATPGroup
5
Base36
4G
Express
Off
ExpressionSys
Sf21 cells
KCode
160
Kinase Type
k
PDF
ZAP70_screening.pdf
(86.99 KB)
Species
Human
TissRec
R