Longname
phosphorylase B kinase
3Letter
PBK
Accession
X80590
Activation
N/A constitutive
Assay Method
PhKγ1 (5-20 mU diluted in 50 mM Na-b-glycerophosphate pH 7.0, 0.1% β-mercaptoethanol) is assayed against phosphorylase b peptide (KRKQISVRGL) in a final volume of 25.5 µl containing 50 mM Tris pH 8.6, 50 mM Na-b-glycerophosphate, 0.04 mM CaCl2, phosphorylase b peptide (0.196 mM), 10 mM magnesium acetate, 0.05 mM [33P-γ-ATP] (500 -1000 cpm/pmole) then incubated for 15 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
50
Base36
0X
Express
Off
ExpressionSys
E. coli
KCode
33
Kinase Type
k
PDF
PBK_5.pdf
(74.98 KB)
Species
Human
TissRec
R