Services | Lipid Kinase Screen

There are over 500 kinases that have been identified in the human kinome comprising 518 protein kinases, and approximately 20 lipid kinases (Manning, et al. 2002. Science 298(5600):1912-34). 

At the International Centre for Kinase Profiling (ICKP) we understand that ascertaining how your compound behaves in relation to the multitude of related enzymes is critical. 

With a Lipid Kinase Screen, we will interrogate your compound against a series of 15 enzymes that represent a variety of key lipid kinase family members.

To be included in the next screen your compounds must arrive at the facility by:

17th July 2017

Submission Guidelines

Download: Compound Submission Form

All customers: Please provide a completed Compound Submission Form.

  • Academic customers are required to read the Terms & Conditions and indicate their agreement on the Compound Submission Form.
  • For-Profit customers are required to complete and return the Overarching Service Agreement before work can commence.

Amount:

  • Dry: Must be soluble in Dimethyl Sulfoxide (DMSO)
  • Wet:  
  • A minimum of 0.15 ml of each compound in 100% DMSO
  • Concentration: 51 times the concentration at which the screen is to be carried out

Number of Compounds:

  • If there are more than 30 compounds please contact the ICKP for additional details regarding submission 

Kinase Panel

Kinase Full Name Species
CHKα [1-457] Choline kinase alpha Human
CHKβ [1-395] Choline kinase beta Human
DGKβ [1-804] Diacylglycerol kinase beta Human
DGKγ [1 -752] Diacylglycerol kinase gamma Human
DGKζ [1 -929] Diacylglycerol kinase zeta Human
PI3Kα E542K [2 - 1068] - p85 [1 - 724] Phosphatidylinositol 3-kinase alpha E542K mutant and Regulatory Subunit 1 Human
PI3Kα E545K [2 - 1068] - p85 [1 - 724] Phosphatidylinositol 3-kinase alpha E545K mutant and Regulatory Subunit 1 Human
PI3Kα [2-1068] - p85 [1-724] Phosphatidylinositol 3-kinase type 1 alpha and Regulatory Subunit 1 Human
PI3Kβ [1-1070] - p85 [1-724] Phosphatidylinositol 3-kinase type 1 beta and Regulatory Subunit 1 Human
PI3Kγ [2-1102] Phosphatidylinositol 3-kinase type 1 gamma Human
PI4K2α [1 - 479] Phosphatidylinositol 4-kinase type 2-alpha Human
PIK4Cβ [1-801] Phosphatidylinositol 4-kinase beta Human
PIP5K2α [1-406] Phosphatidylinositol-5-phosphate 4-kinase type-2 alpha Human
SPHK 1 [1-384] Sphingosine Kinase 1 Human
SPHK 2 [1-654] Sphingosine Kinase 2 Human

Workflow

The method utilized for lipid kinase screening is the Promega ADP Glo High Throughput Assay kit.   The principle of this assay is that  it is a measurement of the ADP generated as a function of the enzyme reaction.

Assay step-by-step process:

1. Upon receipt of your small molecule, staff at the ICKP will dilute each to the appropriate concentration (if required)

2. This compound is added to a ‘mother plate’ consisting of customer samples, controls and blanks

  • These serve as the source for ‘daughter plates’ which are stored at -20* until assay initiation
  • Note: All compounds are screened in duplicate

3. Promega ADP Glo HTS

Data Analysis:

1. Bar codes assigned to each file ensure that data corresponding to the correct compound is being analysed

2. After completion of each assay, ICKP staff ensure that the run has passed standard quality control measures by examining reference compounds on the QC plate

3. Upon determination that the run has met QC standards, a Z-Prime (Z’) value is calculated utilizing data from the controls/blanks on each individual plate

  • This QC measure is in place to ensure that each individual plate in the run has passed QC

4. Finally, a mean percentage activity is calculated for each customer.

  • A standard deviation for all the duplicates is also calculated

 

Each customer receives mean percentage activity and standard deviation for

each compound tested against all enzymes

Timeline

  • This screen is undertaken once every 3 weeks.
  • Data is provided 1 week from the start date.
  • Start dates for each screen can be found here.

Pricing

  • We work with BOTH academia and industry
  • We offer competitive rates.
  • There will be discounts for larger projects (>40 compounds within the same screen)
  • Please contact us to discuss your requirements

Shipping to the UK

In order to minimize customs delays we encourage all customers to provide a sufficient amount of information on each package including:

  • Compound Name(s)
  • Derivation Source (synthetic, natural)
  • Intended Use (e.g. Research)

Shipping Address:

International Centre for Kinase Profiling Division of Signal Transduction Therapy
College of Life Sciences
University of Dundee
Dow Street
Dundee DD1 5EH
United Kingdom
Phone: +44-(0)1382-388-056

Do you need any help? Please get in touch and we’ll be happy to lend a hand.