Services | Substrate Screen

There are over 500 kinases that have been identified in the human kinome comprising 518 protein kinases, and approximately 20 lipid kinases (Manning, et al. 2002. Science 298(5600):1912-34). 

At the International Centre for Kinase Profiling (ICKP) we are happy to help you with all steps of your assay to provide you with the key information that can help move your studies forward. 

While the enzymatic action of known kinases involves phosphorylation of target proteins, for many of these kinases, the physiological substrate has yet to be identified.  In these cases, we can help customers to identify an optimal substrate that may be useful for subsequent in vitro studies.  In this way, one can examine the kinetics of substrate phosphorylation at multiple enzyme concentrations and also examine how compound(s) of interest affect phosphorylation state using these ‘place-holder’ substrates.  Such a screen may yield valuable clues about your enzyme of interest.

Submission Guidelines

Critical to these studies is that the customer can provide their enzyme of interest at the appropriate concentrations and volumes

  • Please note that we routinely express and purify proteins in vitro from bacterial and insect cell systems.  If you need assistance with generation and purification of your enzyme of interest, please get in touch!

Please contact the ICKP to discuss these details so that we may assist you

in planning the most informative study possible

Critical to these studies is that the customer can provide their enzyme of interest at the appropriate concentrations and volumes

  • Please note that we routinely express and purify proteins in vitro from bacterial and insect cell systems.  If you need assistance with generation and purification of your enzyme of interest, please get in touch!

Please contact the ICKP to discuss these details so that we may assist you

in planning the most informative study possible

Workflow

Greater than 94% of the 140 enzymes in our premier screen panel are assayed using a selection of 42 peptide substrates and 2 protein substrates.  We can work with your purified enzyme of interest and screen this this against our panel of peptide and protein substrates and compare with your usual substrate if applicable.  Additionally, follow up substrate Km measurements can also be carried out.

Please contact us to discuss further details, analysis requirements and pricing.

Timeline

  • Rapid turnaround times are possible with these screens (1 week or less)
  • Please contact us to discuss your needs

Pricing

  • We work with BOTH academia and industry
  • We offer competitive rates
  • Please contact us to discuss your requirements

Shipping to the UK

In order to minimize customs delays we encourage all customers to provide a sufficient amount of information on each package including:

  • Compound Name(s)
  • Derivation Source (synthetic, natural)
  • Intended Use (e.g. Research)

Shipping Address:

International Centre for Kinase Profiling Division of Signal Transduction Therapy
College of Life Sciences
University of Dundee
Dow Street
Dundee DD1 5EH
United Kingdom
Phone: +44-(0)1382-388-056

Do you need any help? Please get in touch and we’ll be happy to lend a hand.