Longname
AMP-activated protein kinase
3Letter
AMP
Accession
NM_006251.5, NM_005399.3, BC000358.2
Activation
N/A constitutive
Assay Method
AMPK (5–20 mU diluted in 50 mM Hepes pH 7.5, 1 mM DTT, 0.02% Brij35) is assayed against SAMS substrate peptide (HMRSAMSGLHLVKRR) in a final volume of 25.5 µl containing 50 mM Hepes pH 7.5, 1 mM DTT, 0.02% Brij35, 0.4 mM SAMS peptide, 0.196 mM AMP, 10 mM magnesium acetate and 0.02 mM [33P-g-ATP] (50-1000 cpm/pmole) and incubated for 30 min at room temperature. Assays are stopped by addition of 5 µl of 0.5 M (3%) orthophosphoric acid and then harvested onto P81 Unifilter plates with a wash buffer of 50 mM orthophosphoric acid.
ATPGroup
20
Base36
56
Express
On
ExpressionSys
Sf21 cells
KCode
186
Kinase Type
k
PDF
AMPKA1_-_B2_-_G1_screening.pdf
(95.23 KB)
Species
Human
TissRec
R