Inhibitor | Akti 1/2  

Screen Conc: 1.00µM

Kinase Sort descending	% Activity Remaining	Standard Deviation
AMPK	87	6
ASK1	109	3
Aurora A	94	4
Aurora B	100	10
BRSK1	94	4
BRSK2	95	5
BTK	75	2
CAMK1	44	7
CAMKK beta	104	1
CDK2-Cyclin A	108	14
CHK1	98	10
CHK2	89	11
CK1 delta	94	0
CK2	111	0
CLK2	96	10
CSK	102	2
DAPK1	91	4
DYRK1A	84	2
DYRK2	88	5
DYRK3	91	11
EF2K	61	6
EPH-A2	103	6
EPH-A4	101	1
EPH-B1	100	8
EPH-B2	100	8
EPH-B3	96	14
EPH-B4	95	13
ERK1	92	8
ERK2	103	4
ERK8	87	4
FGF-R1	80	7
GCK	89	2
GSK3 beta	103	1
HER4	95	4
HIPK1	77	2
HIPK2	81	4
HIPK3	95	8
IGF-1R	96	0
IKK beta	93	8
IKK epsilon	76	3
IR	109	3
IRAK4	93	6
IRR	94	3
JAK2	103	2
JNK1	90	2
JNK2	92	11
JNK3	104	9
Lck	90	4
LKB1	89	0
MAPKAP-K2	91	3
MAPKAP-K3	88	2
MARK1	79	1
MARK2	93	3
MARK3	98	5
MARK4	103	3
MEKK1	98	2
MELK	79	2
MINK1	54	9
MKK1	84	12
MKK2	101	3
MKK6	101	11
MLK1	73	0
MLK3	89	4
MNK1	110	2
MNK2	100	2
MSK1	94	4
MST2	66	1
MST4	83	2
NEK2a	90	3
NEK6	117	8
NUAK1	71	0
p38 alpha MAPK	82	1
p38 beta MAPK	108	9
p38 delta MAPK	88	8
p38 gamma MAPK	97	3
PAK2	92	8
PAK4	68	9
PAK5	63	1
PAK6	109	8
PDK1	108	4
PHK	102	11
PIM1	80	0
PIM2	86	1
PIM3	63	1
PKA	100	10
PKB alpha	95	4
PKB beta	65	7
PKC alpha	82	3
PKC zeta	113	4
PKD1	86	7
PLK1	98	4
PRAK	92	5
PRK2	79	7
RIPK2	57	3
ROCK 2	82	10
RSK1	97	4
RSK2	74	1
S6K1	106	8
SGK1	98	4
SmMLCK	82	13
Src	105	9
SRPK1	84	7
SYK	89	7
TAK1	78	8
TAO1	87	4
TBK1	88	4
TrkA	76	7
TTK	92	3
VEGFR1	71	3
YES1	79	6

Screen Conc: 0.10µM

Kinase Sort descending	% Activity Remaining	Standard Deviation
AMPK	96	14
Aurora B	83	5
Aurora C	83	0
BRSK2	93	0
CAMK1	90	3
CDK2-Cyclin A	92	3
CHK1	97	0
CHK2	83	7
CK1 delta	106	7
CK2	85	1
CSK	97	11
DYRK1A	97	11
DYRK2	105	3
DYRK3	87	6
EF2K	107	11
EPH-A2	95	6
ERK1	95	3
ERK2	71	15
ERK8	80	5
FGF-R1	89	8
GSK3 beta	83	3
HIPK2	98	6
HIPK3	72	2
IKK beta	91	11
IKK epsilon	107	7
IRR	91	3
JNK1	85	0
JNK2	95	0
JNK3	79	3
Lck	81	4
MAPKAP-K2	84	6
MAPKAP-K3	76	3
MARK3	86	1
MELK	86	10
MKK1	75	2
MNK1	81	0
MNK2	93	11
MSK1	84	2
MST2	103	0
MST4	92	1
NEK2a	92	6
NEK6	83	1
NEK7	86	0
p38 alpha MAPK	111	5
p38 beta MAPK	103	10
p38 delta MAPK	104	5
p38 gamma MAPK	110	7
PAK4	99	4
PAK5	87	7
PAK6	112	8
PDK1	91	9
PHK	104	1
PIM1	83	12
PIM2	95	6
PIM3	97	5
PKA	90	8
PKB alpha	85	3
PKB beta	101	6
PKC alpha	101	5
PKC zeta	101	0
PKD1	90	4
PLK1	83	2
PRAK	93	5
PRK2	78	1
ROCK 2	92	7
RSK1	94	4
RSK2	85	7
S6K1	98	1
SGK1	106	5
SmMLCK	86	7
Src	97	3
SRPK1	87	1
SYK	83	2
TBK1	103	11
YES1	86	4

Screen Conc: 100.00µM

Kinase Sort descending	% Activity Remaining	Standard Deviation
AMPK	97	0
Aurora B	56	11
Aurora C	70	15
BRSK2	76	9
CAMK1	8	0
CDK2-Cyclin A	93	3
CHK1	88	5
CHK2	58	3
CK1 delta	88	5
CK2	84	7
CSK	92	0
DYRK1A	99	5
DYRK2	106	0
DYRK3	60	4
EF2K	43	1
EPH-A2	69	1
ERK1	87	2
ERK2	45	1
ERK8	51	1
FGF-R1	55	0
GSK3 beta	43	9
HIPK2	61	1
HIPK3	68	2
IKK beta	98	2
IKK epsilon	100	1
IRR	68	1
JNK1	48	1
JNK2	80	7
JNK3	42	1
Lck	31	3
MAPKAP-K2	12	0
MAPKAP-K3	52	2
MARK3	90	8
MELK	45	5
MKK1	14	3
MNK1	82	0
MNK2	77	10
MSK1	41	1
MST2	83	7
MST4	92	6
NEK2a	70	2
NEK6	44	1
NEK7	15	0
p38 alpha MAPK	83	2
p38 beta MAPK	86	15
p38 delta MAPK	89	3
p38 gamma MAPK	87	0
PAK4	33	1
PAK5	63	5
PAK6	66	1
PDK1	98	15
PHK	59	2
PIM1	17	1
PIM2	23	0
PIM3	16	1
PKA	39	0
PKB alpha	6	0
PKB beta	26	2
PKC alpha	96	3
PKC zeta	104	8
PKD1	81	3
PLK1	83	2
PRAK	51	4
PRK2	59	2
ROCK 2	92	9
RSK1	36	1
RSK2	22	1
S6K1	34	11
SGK1	14	5
SmMLCK	-1	0
Src	62	5
SRPK1	67	0
SYK	44	2
TBK1	100	4
YES1	61	2

Screen Conc: 10.00µM

Kinase Sort descending	% Activity Remaining	Standard Deviation
AMPK	92	10
Aurora B	60	3
Aurora C	65	0
BRSK2	92	12
CAMK1	3	0
CAMKK alpha	62	3
CAMKK beta	108	9
CDK2-Cyclin A	97	13
CHK1	85	2
CHK2	82	7
CK1 delta	110	2
CK2	91	14
CSK	103	15
DYRK1A	93	12
DYRK2	118	1
DYRK3	74	1
EF2K	40	6
EPH-A2	84	7
ERK1	99	5
ERK2	75	14
ERK8	70	1
FGF-R1	75	3
GSK3 beta	77	9
HIPK2	84	3
HIPK3	65	2
IKK beta	89	2
IKK epsilon	67	6
IRR	81	13
JNK1	70	9
JNK2	81	12
JNK3	80	7
Lck	64	6
MAPKAP-K2	59	1
MAPKAP-K3	71	5
MARK3	95	12
MELK	67	0
MKK1	76	10
MNK1	84	1
MNK2	79	3
MSK1	93	14
MST2	78	2
MST4	80	4
NEK2a	99	3
NEK6	75	3
NEK7	84	10
p38 alpha MAPK	98	13
p38 beta MAPK	113	3
p38 delta MAPK	98	11
p38 gamma MAPK	93	9
PAK4	48	4
PAK5	60	3
PAK6	92	1
PDK1	94	7
PHK	101	9
PIM1	56	6
PIM2	89	3
PIM3	59	6
PKA	76	5
PKB alpha	64	8
PKB beta	87	14
PKC alpha	87	8
PKC zeta	96	9
PKD1	82	14
PLK1	89	2
PRAK	79	5
PRK2	67	10
ROCK 2	91	1
RSK1	67	1
RSK2	74	1
S6K1	71	6
SGK1	82	3
SmMLCK	11	4
Src	118	1
SRPK1	84	9
SYK	84	10
TBK1	106	10
YES1	88	8